An experiment was carried out to investigate the performance of young steers given either ammoniated hay alone (TA), or supplemented daily with either 112 g fish meal (TF) or 2 kg barley (TB) or 2 kg barley-based concentrate (TC), compared with untreated hay supplemented with either urea (UU), to raise the nitrogen content of the diet to that of the ammoniated hay, or with 2 kg concentrate (UC). Serial slaughter of animals was carried out to examine the effects on body composition during the winter feeding period and subsequent grazing season.

Ammoniation of the hay significantly increased the in vivo digestibility of organic matter, acid-detergent fibre, neutral-detergent fibre and cellulose fractions (0·672 v. 0·643, 0·740 v. 0·670, 0·758 v. 0·638 and 0·826 v. 0·741, respectively). When offered with 2 kg concentrate, intake of ammoniated hay was higher than that of the untreated hay. Empty body weight gains (EBWG) during the winter period were affected by treatment (174, 225, 293, 590, 631 and 692 g/day for treatments UU, TA, TF, UC, TB and TC, respectively), and were highly correlated with total gross energy intakes (56·0, 61·0, 62·9, 74·5, 73·2 and 83·1 MJ/day, respectively). As the rate of EBWG increased, the fat proportion (g/kg EBW) at turn-out also increased. During the grazing season animals which had been on treatments UU, TA and TF showed a marked degree of compensatory growth (759, 686 and 640 g/day EBWG, respectively) compared with treatments UC, TB and TC (623, 572 and 601 g/day EBWG, respectively), and differential rates of tissue gain. This resulted in reduced, though still significant, differences in EBW, although body composition (g/kg EBW) at final slaughter was similar for all treatments. Thus, whilst short-term advantages in intake and performance can be obtained by ammoniation of hay, the benefits so achieved may not persist due to subsequent compensatory growth at pasture.

Commercial application of multiple ovulation and embryo transfer (MOET) technology will be subject to practical constraints and economic rationalism. This study examines use of MOET in its most profitable arena: to breed stud rams which will disseminate genetic improvement widely through multiplier studs to commercial flocks. A deterministic prediction is used to evaluate schemes based on an open nucleus MOET group within a Merino parent stud, taking account of genetic merit and inbreeding. Selection is based on clean fleece weight with an assumed heritability of 0·4. Embryos are collected at a rate equivalent to 3·45 live lambs per donor. Benefits of MOET were calculated from the discounted expressions of rams sold, and compared with the costs incurred.

As the proportion of the flock born from MOET increases, the rate of genetic gain increases rapidly at first, but diminishing returns are observed. The costs ofMOET increase linearly with the number of lambs produced, so the optimum proportion ofMOET lambs is for practical purposes always less than 100%.

Some use of MOET was profitable provided the stud sells sufficient stud rams each year. Sensitivity tests found that other parameters had only a small impact on the optimum level ofMOET. In general however, changes which increased the rate of genetic gain (heritability, flock size) or increased its value (wool price, lower discount rate) increased the optimum number ofMOET lambs.

The results should provide guidelines to optimum investment in MOET for the wool industry. An across flock genetic evaluation scheme is probably necessary to motivate this investment.

Forty-eight pregnant Manchega dairy ewes bearing twins or singles penned in groups of six during the last 11 weeks of gestation were used to study the effects offorage type (Italian ryegrass hay or alfalfa hay) and number of foetuses (one or two) on voluntary dry-matter (DM) intake and digesta kinetics. Hays were supplemented with 0·6 kg/day of concentrate. When measured directly for group-fed ewes, forage DM intake was affected byforage type (P < 0·01), whereas litter size had no significant effect. An internal (rumen-undegradable DM) and an external (chromium III oxide) marker were also used to determine intake of individual ewes to account for errors in diagnosing litter size. Estimated DM intake confirmed the effect offorage type on DM intake and revealed the effect of litter size on food intake (F < 0·05). Forage type also affected chromium III oxide fractional outflow rate and flow of undegradable DM, whereas litter size only had an effect at the end of pregnancy by reducing hay intake, rate of passage and digesta flow mainly with alfalfa diets. In conclusion, DM intake, rate of passage and digesta flow was higher with alfalfa hay than with ryegrass hay, in spite of the similar DM apparent digestibilities of both hays. Furthermore, litter size affected DM intake at the end of pregnancy causing an intake decline, which was greater with alfalfa hay than with ryegrass hay.

Two trials were conducted with two complete fattening diets contain- ing 25 and 45 % fibrous roughage, to examine the effects of three moisture levels and five soaking conditions on the performance and ruminal fermentation of fattening cattle.

Differences between treatments in live-weight gain and carcass-weight gain were not significant but there were differences in the efficiency of conversion of metabolizable energy into live weight and into carcass weight and in some instances these were significant. There was a trend towards increased weight of kidney, pelvic and cod fats and a slight decrease in weight of other fat trim of the carcass (subcutaneous and intermuscular) on the treated diets, as compared with the dry control diet.

The pH of the rumen liquor of animals given the soaked rations was lower and the total volatile fatty acid concentration higher than that of animals receiving the dry control diet. The propionic acid concentration in rumen liquor was significantly higher in animals receiving moistened diets.

The first paper of this series (Alder, Tayler and Rudman, 1964) described experiments carried out over 4 years on the effects of hexoestrol on live-weight gain and herbage intake in grazing steers. The 52 steers used weighed between 750 and 1,100 lb. initially and included four pairs of twins. The experiments lasted between 55 and 124 days and involved both summer and winter grazing. This paper is concerned with observations made on the animals after slaughter.

Daily partial milk yield of Sanga cows in 13 smallholder agropastoralist herds at two locations on the Accra Plains was recorded through monthly sampling. Estimates of mean daily partial milk yield and milk composition were computed from 1266 observations. The unadjusted mean daily partial milk yield was 875 (s.e. 11) g. The composition of milk: total solids (TS), fat (F) and solid-not-fat (SNF) were 131 (s.e. 14), 41 (s.e. 11) and 90 (s.e. 7) g/kg respectively. Least squares estimate of mean daily partial milk yield were 808·2 and 462·4 g with average s.e. of 41·8 for wet and dry season respectively. The wet and dry season differences in daily partial milk yield, TS and F were highly significant (P < 0·01). Seasonal difference in SNF was not significant.

Herd difference in partial milk yield, TS, F and SNF were highly significant (P < 0·02). There were no significant effects of location on daily partial milk yield and SNF, although location effects on F and TS were significant (P < 0·05). The highest milk yield was observed in cows in third lactation and the lowest in cows in seventh lactation (P < 0·01). Daily partial milk yield peaked in the 3rd month of lactation. On average, lactation length was 210 to 240 days. Both linear and quadratic effects of stage of lactation were highly significant (P < 0·01). Lactation curve parameters for partially milked cows were also estimated. The implications of the results for milk production in sub-Saharan Africa were discussed.

In three experiments, seven grass silages, four grass hays and winter barley straw, with and without anhydrous ammonia treatment, were offered ad libitum to a total of 134 Finn Dorset ewes in weeks 11 to 15 of pregnancy and intakes were measured. The untreated straw diet was supplemented with 300 g fresh weight of concentrates per ewe. Number of foetuses carried did not affect intakes in mid pregnancy. Organic matter digestibilities measured in vivo in week 15, ranged from 0·39 to 0·69 for hays and 0·53 to 0·79 for silages. Generally, between weeks 11 to 15 of pregnancy, hay intakes showed either little change or slight increases whereas intakes of silages or straws showed little change or slight decreases. Forage intake was higher on the better quality hays and silages and ewes gained weight and body condition, compared with losses for the poorer diets. In late pregnancy, the ewes were offered grass silage ad libitum and rations of concentrates according to a rationing programme. There were no residual effects of the mid-pregnancy treatments on lamb birth weights but ewes offered the poorer diets in mid pregnancy still tended to be lighter and thinner post partum. Equations are presented to predict the intakes of hays and silages from food and animal factors.

There have been suggestions that positive feedback from the meal may stimulate the performance of undesirable behaviour, such as stereotypies, in the post-feeding period. The aim of this work was to measure the short-term effects of a meal on the post-feeding behaviour of the pig.

Pigs (70 kg live weight) were offered food ad libitum for 7 days and then given a restricted food allowance for 23 days (experiment 1) or 14 days (experiment 2). In experiment 1, the food allowance was given in one, two or three meals per day. In experiment 2, the food allowance was given in two meals per day and comprised ingredients which supplied glucose at different rates (starch, glucose/starch, starch/guar gum). The pigs' posture and behaviour were recorded in three 1-h periods during the day on 2 days consecutively during the ad libitum feeding period (time period 1) and on pairs of days on three (periods 2, 3 and 4: experiment 1) or two (periods 2 and 3: experiment 2) subsequent occasions during food restriction. The meal frequency or estimated rate of glucose supply had no significant effects on posture or behaviour. In both experiments activity increased with the duration of food restriction. In periods 1, 2, 3 and 4 of experiment 1 the proportions of observations spent were: standing 0·244, 0·260, 0·706 and 0·728 (s.e.d. 0·0346); nosing 0·073, 0·094, 0·229 and 0·253 (s.e.d. 0·0169); rooting 0·032, 0·037, 0·108 and 0·133 (s.e.d. 0·0133). In periods 1, 2 and 3 of experiment 2 the proportions were: standing 0·247, 0·302 and 0·446 (s.e.d. 0·0240); nosing 0·045, 0·075 and 0·138 (s.e.d. 0·0113); chewing 0·101, 0·126, 0·155 (s.e.d. 0·0121). In experiment 1 feeding rate increased with duration of restriction (P < 0·001), but was unaffected by treatment. It is concluded that post-feeding behaviour was influenced more by long-term changes in state (i.e. prolonged food restriction) than by short-term effects of the meal.

In the dairy industry, excess dietary CP is consistently correlated with decreased conception rates. However, amount of excess CP effects on reproductive function in beef cattle is largely undefined. The objective of this experiment was to determine the effects of excess metabolizable protein (MP) supplementation from a moderately abundant rumen undegradable protein (RUP) source (corn gluten meal: 62% RUP) on ovarian function and circulating amino acid (AA) concentrations in beef cows consuming low quality forage. Non-pregnant, non-lactating beef cows (n=16) were allocated by age, BW and body condition score (BCS) to 1 of 2 isocaloric supplements designed to maintain BW for 60 days. Cows had ad libitum access to corn stalks and were individually offered a corn gluten meal-based supplement daily at 125% (MP125) or 150% (MP150) of National Research Council (NRC) MP requirements. After a 20-day supplement adaptation period, cows were synchronized for ovulation. After 10 days of synchronization, follicular growth was reset with gonadotropin releasing hormone. Daily thereafter, transrectal ultrasonography was performed to diagram ovarian follicular waves, and blood samples were collected for hormone, metabolite and AA analyses. After 7 days of observation of estrus, corpus luteum (CL) size was determined via ultrasound. Data were analyzed using the MIXED procedures of SAS. No differences (P⩾0.21) in BW and BCS existed throughout the study; however, plasma urea N at ovulation was greater (P=0.04) in MP150. Preovulatory ovarian follicle size at dominance, duration of dominance, size at spontaneous luteolysis, length of proestrus and wavelength were not different (P⩾0.11) between treatments. However, ovulatory follicles were larger (P=0.04) and average antral follicle count was greater (P=0.01) in MP150 than MP125. Estradiol concentration and ratio of estradiol to ovulatory follicle volume were not different due to treatment (P⩾0.25). While CL volume 7 days post-estrus was greater (P<0.01) in MP150 than MP125, circulating progesterone 7 days post-estrus and ratio of progesterone to CL volume were not different (P⩾0.21). Total AA were not different (P⩾0.76) at study initiation or completion; however, as a percent of total AA, branched-chain AA at ovulation were greater (P=0.02) in MP150. In conclusion, supplementation of CP at 150% of NRC MP requirements from a moderately undegradable protein source may enhance growth of the ovulatory follicle and subsequent CL compared with MP supplementation at 125% of NRC MP requirements.

The objective of this study was to further the understanding of the effects of dietary protein and energy supplements on growth, performance, feed intake and grass forage digestibility in growing meat goat wethers. In Experiment 1, an 18% CP complete goat pellet was offered alone (control diet, C) or added (+), or not, as supplement to three grass hays (coastal bermudagrass, CB; Tifton 85 bermudagrass, T; and sorghum-Sudan grass hay, SS), to Boer-cross wethers (n = 72). The resulting seven diets were offered ad libitum. In Experiment 2, four wether goats in metabolism crates were used in a 4 × 4 Latin square design and fed a SS basal diet ad libitum with treatments consisting of no supplement, supplemental urea (200 mg/kg BW daily), supplemental dextrose (0.2% BW daily), or urea + dextrose (200 mg/kg BW daily and 0.2% BW daily, respectively). In Experiment 1, average daily gain (ADG) were −3.8, −5.0 and −6.6 g/day for goats consuming CB, T and SS, respectively, and 69.2, 61.6 and 58.1 g/day for supplemented CB (CB+), T (T+) and SS (SS+), respectively, as compared to 245.8 g/day for ad libitum access to C. Supplementation in Experiment 1 increased (P < 0.01) ADG for all hays when compared to hay-only diets. In Experiment 2, protein and energy supplementation increased (P < 0.01) nitrogen retention but did not impact diet digestibility. The beneficial effects of supplements in Experiment 1 and the increase in nitrogen retention in Experiment 2 cannot be explained by improvements in ruminal fiber utilization, but could be due to post-ruminal nutrient supply and/or increased ruminal microbial protein synthesis.

Fully slatted concrete floors are labour-efficient, cost-effective and thus common in beef cattle housing. However, the welfare of cattle accommodated on them has been questioned. The objective of this study was to evaluate the effect of floor and diet on hoof health and lying behaviours of housed dairy-origin bulls, from a mean age of 8 months to slaughter at 15.5 months old. Forty-eight bulls, which had a mean initial live weight of 212 (SD = 23.7) kg, were allocated to one of four treatments, which consisted of two floors and two diets arranged in a 2 × 2 factorial design. The floors evaluated were a fully slatted concrete floor and a fully slatted concrete floor overlaid with rubber, while the diets offered were either a high concentrate diet or a grass-silage-based diet supplemented with concentrates. The mean total duration of the study was 216 days. Floor had no significant effect on claw measurements measured on day 62 or 139. However, bulls accommodated on slats overlaid with rubber had a tendency to have a higher front toe length measured pre-slaughter than those accommodated on concrete slats (P = 0.063). Floor had no significant effect on the net growth of toes or heels during the duration of the study. The number of bruises (P < 0.01) and the bruising score (P < 0.05) were significantly higher on day 62 in bulls accommodated on fully slatted concrete floors than on concrete slats overlaid with rubber, but there was no significant effect of floor on these parameters on day 139 or at the measurement taken pre-slaughter. There was a tendency for bulls accommodated on concrete slats to have a higher probability of having sole bruising at the end of the experiment than those accommodated on slats overlaid with rubber (P = 0.052). Diet had no significant effect on toe length or heel height, number of bruises, or overall bruising score at any time point of the study. There was little evidence in the current study to suggest that bulls lying on fully slatted concrete floors could not express lying postures similar to those on concrete slats overlaid with rubber.

H2A histone family, member Z (H2A.Z) is required for early mammalian development. In the present study, the 932 bp of full-length cDNA encoding a 128 amino-acid protein and the sequences of intron 2 to 4 of the porcine H2A histone family, member Z (pH2AFZ) gene were obtained. By comparative sequencing of pH2AFZ gene in Large White and Meishan pigs, a 4 bp deletion/insertion in intron 2 was detected and a PCR-Bsu15I-RFLP was established to detect this variation. In DIV (4th Dam line of Chinese lean-type new lines) pigs, the first-parity females with AA genotype had fewer piglets born alive (−2.64 and −1.83 piglets per litter) than those with AB (P < 0.01) and BB (P < 0.05) genotype. The additive allelic and dominance effect were estimated to be 0.92 (P < 0.05) and −0.87 piglets per litter (P < 0.01) for number of piglets born alive, respectively. This result suggests that the pH2AFZ gene might be a good candidate gene of litter-size trait and provides some marker information for marker-assisted selection.

Early lactation parameters are difficult to estimate from commercial dairy records due to the small number of records available before the peak of production. A biological model of lactation was used with weekly milk records from a single Holstein herd to estimate these early lactation parameters and the secretion rate of milk from the average cell throughout lactation. A genetic analysis of the lactation curve parameters, calculated curve characteristics and secretion rate traits was undertaken. Early lactation traits were found to have little genetic variation and effectively zero heritability. Secretion rate traits for milk, protein, lactose and water were all moderately heritable and highly genetically correlated (>0.87) but fat secretion rate had lower genetic correlations with the other secretion rates. A similar pattern of correlations was seen between total lactation yield traits for fat, protein, lactose and water. The genetic correlations between the lactation curve traits and the secretion rate traits were calculated. Total milk yield, peak yield and maximum secretion potential were all highly correlated with milk, lactose and water secretion rates but less so with fat and protein secretion rates. In particular, fat secretion rate had a moderate to low genetic correlation with these lactation curve traits. Persistency of lactation was highly correlated with fat and protein secretion rates, more persistent lactations being associated with lower rates of secretion of these milk components. Similar levels of heritability were found, where trait genetic parameters were directly equivalent to those derived from the same dataset by random regression methods. However, by using a biological model of lactation to analyse lactation traits new insights into the biology of lactation are possible and ways to select cows on a range of lactation traits may be achieved.

Energy reserve, estimated as body condition score (BCS), is the major determinant of the re-initiation of ovarian activity in postpartum cows. Leptin, IGF-I and insulin are positively related to BCS and are putative mediators between BCS and reproductive function. However, when BCS and body composition dissociates, concentrations of these metabolic hormones are altered. We hypothesized that increasing lean muscle tissue, but not fat tissue, would diminish the reproductive response to oestrus induction treatments. Thirty lactating beef cows with BCS of 3.10±1.21 and 75.94±12 days postpartum were divided in two groups. Control cows (n=15) were supplemented with 10.20 kg of concentrate daily for 60 days. Treated cows (n=15) were supplemented equally, and received a β-adrenergic receptor agonist (β-AA; 0.15 mg/kg BW) to achieve accretion of lean tissue mass and not fat tissue mass. Twelve days after ending concentrate supplementation/β-AA treatment, cows received a progestin implant to induce oestrus. Cows displaying oestrus were inseminated during the following 60 days, and maintained with a fertile bull for a further 21 days. Cows in both groups gained weight during the supplementation period (Daily weight gain: Control=0.75 kg v. β-AA=0.89 kg). Cows treated with β-AA had a larger increase in BCS (i.e. change in BCS: control=1 point (score 4.13) v. β-AA=2 points (score 5.06; P<0.05), as a result of muscle accretion (i.e. change in muscle depth: control 0.21 cm v. β-AA 0.97 cm; P<0.05) but not adipose tissue (i.e. change in back fat depth; control 0.13 cm v. β-AA −0.06 cm; P<0.05). The changes in body composition in β-AA cows were associated with a reduction in serum concentrations of IGF-I (25.4%) and leptin (27.9%), without observed changes in insulin. Ovulation and pregnancy to 1st service (P>0.05) did not differ between groups. However, the number of cows displaying oestrus (control 13/15 v. β-AA 8/15; P<0.05) and the percentage cycling (control 6/8 v. β-AA 3/10; P=0.07) after progestin treatment and the pregnancy percentage at the end of the breeding period (control 13/15 v. β-AA 8/15; P<0.05) were lower in β-AA than control cows. In summary, the increase BCS through muscle tissue accretion, but not through fat tissue accretion, resulted in a lower response to oestrus induction, lower percentage of cycling animals and lower pregnancy percentage after progestin treatment; which was associated with a decrease in serum concentrations of leptin and IGF-I.

In sows, n-3 fatty acids increase litter sizes, however, effects on gilt reproductive development have not been adequately studied. Moreover, not determined are effects of feeding n-3 fatty acids to sows on reproduction in offspring. The objective here was to determine effects of 4% dietary menhaden oil on growth and puberty in gilts farrowed by sows fed menhaden oil. Sows (n = 44) were assigned to: (1) control gestation and lactation diets, or (2) diets including menhaden oil. For primiparous sows only, total litter size and born alive were greater (P < 0.05) in females fed menhaden oil. Conversely, pigs from primiparous controls were heavier (P < 0.05) than pigs from primiparous sows fed menhaden oil (parity by diet interactions, P < 0.01). Diet did not affect (P > 0.20) other sow and litter characteristics. At weaning, 84 gilts from control- or menhaden oil sows were placed three gilts per pen and provided control diets or diets containing menhaden oil. Nursery and grow-finish feed intake and feed efficiency were similar (P > 0.21) for gilts from the different sows and weight gain was similar (P > 0.24) for gilts fed control or menhaden diets. Gilts fed menhaden oil tended to eat less in the nursery (1.18±0.08 kg v. 0.98±0.08 kg; P = 0.09) and overall (1.83±0.04 kg v. 1.72±0.04 kg; P = 0.06). Thus, overall feed to gain was greater (2.52±0.03 v. 2.33±0.03; P < 0.01) and nursery (2.12±0.04 v. 1.80±0.04; P = 0.10) and grow-finish (3.07±0.19 v. 2.58±0.19; P = 0.08) feed to gain tended to be greater, for control gilts. Age at puberty was greater (P = 0.02) for gilts from menhaden oil-fed sows (205.1±3.2 days) compared to gilts from controls (193.9±3.2 days) and tended to be greater (P = 0.09), for controls (203.5±3.2 days) compared to gilts fed menhaden oil (195.5±3.2 days). A tendency existed (P = 0.09) for greater follicular fluid in gilts fed menhaden oil, however, ovulation rate and ovarian, luteal and uterine weights were not affected by sow diet, gilt diet or the interaction (P > 0.23). Feeding gilts menhaden oil enhanced feed efficiency and hastened puberty onset. Gilts from sows consuming menhaden oil exhibited delayed puberty and retaining females from sows fed this feedstuff may be ill advised.

The objective of this study was to carry out a sensitivity analysis on the impact of various production strategies and performance levels on the relative economic values (REVs) of traits in dairy sheep. A bio-economic model implemented in the program package ECOWEIGHT was used to simulate the profit function for a semi-extensive production system with the Slovak multi-purpose breed Improved Valachian and to calculate the REV of 14 production and functional traits. The following production strategies were analysed: differing proportions of milk processed to cheese, customary weaning and early weaning of lambs with immediate sale or sale after artificial rearing, seasonal lambing in winter and aseasonal lambing in autumn. Results of the sensitivity analysis are presented in detail for the four economically most important traits: 150 days milk yield, conception rate of ewes, litter size and ewe productive lifetime. Impacts of the differences in the mean value of each of these four traits on REVs of all other traits were also examined. Simulated changes in the production circumstances had a higher impact on the REV for milk yield than on REVs of the other traits investigated. The proportion of milk processed to cheese, weaning management strategy for lambs and level of milk yield were the main factors influencing the REV of milk yield. The REVs for conception rate of ewes were highly sensitive to the current mean level of the trait. The REV of ewe productive lifetime was most sensitive to variation in ewe conception rate, and the REV of litter size was most affected by weaning strategy for lambs. On the basis of the results of sensitivity analyses, it is recommended that economic values of traits for the overall breeding objective for dairy sheep be calculated as the weighted average of the economic values obtained for the most common production strategies of Slovak dairy sheep farms and that economic values be adjusted after substantial changes in performance levels of the traits.

Salernitano (SAL) is an ancient Italian horse breed developed over the course of the ages together with Napoletano and, during the 20th century, by crossing with Thoroughbred horse lines. Excellent in hurdle jumping, this breed is currently facing a concrete risk of extinction due to the lack of appropriate management strategies. This research is the first SAL genetic characterization that aims to set up the basic knowledge for a conservation plan. A representative sample of 61 SALs was analyzed by means of a set of 16 microsatellites markers (short tandem repeats (STRs)). The sequence of hypervariable D-loop mtDNA region was also performed on a subset of 24 mares in order to study the maternal diversity and obtain a complete picture of the internal genetic variation. All the molecular data were analyzed together with those obtained from three Sicilian horse breeds investigated in a previous research (Siciliano, Sanfratellano and Sicilian Oriental Purebred). STRs markers revealed a moderate level of genetic diversity in SAL (alleles/locus 5.1, He 0.67) and confirmed the hunch of genetic erosion. Autosomal variability highlighted a very light deficit of homozygotes (FIS=−0.067). Experimental D-loop sequences were compared by multiple alignments with those retrieved from biological databases and revealed two unreported haplotypes. The phylogenetic network, which was built on mtDNA sequences, included various cosmopolitan and European horses and showed SAL haplotypes distributed among different mtDNA lineages.

The aim of this paper was to study the relationship between milk flow emission variables recorded during milking of dairy goats with variables related to milking routine, goat physiology, milking parameters and milking machine characteristics, to determine the variables affecting milking performance and help the goat industry pinpoint farm and milking practices that improve milking performance. In total, 19 farms were visited once during the evening milking. Milking parameters (vacuum level (VL), pulsation ratio and pulsation rate, vacuum drop), milk emission flow variables (milking time, milk yield, maximum milk flow (MMF), average milk flow (AVMF), time until 500 g/min milk flow is established (TS500)), doe characteristics of 8 to 10 goats/farm (breed, days in milk and parity), milking practices (overmilking, overstripping, pre-lag time) and milking machine characteristics (line height, presence of claw) were recorded on every farm. The relationships between recorded variables and farm were analysed by a one-way ANOVA analysis. The relationships of milk yield, MMF, milking time and TS500 with goat physiology, milking routine, milking parameters and milking machine design were analysed using a linear mixed model, considering the farm as the random effect. Farm was significant (P<0.05) in all the studied variables. Milk emission flow variables were similar to those recommended in scientific studies. Milking parameters were adequate in most of the farms, being similar to those recommended in scientific studies. Few milking parameters and milking machine characteristics affected the tested variables: average vacuum level only showed tendency on MMF, and milk pipeline height on TS500. Milk yield (MY) was mainly affected by parity, as the interaction of days in milk with parity was also significant. Milking time was mainly affected by milk yield and breed. Also significant were parity, the interaction of days in milk with parity and overstripping, whereas overmilking showed a slight tendency. We concluded that most of the studied variables were mainly related to goat physiology characteristics, as the effects of milking parameters and milking machine characteristics were scarce.