There are important differences in terms of metabolic activity, energy utilization and capacity of protein and fat deposition when Iberian and modern pigs are compared. Primary culture of hepatocytes was used to evaluate hepatic function and sensitivity to hormones between breeds without the interference of circulating blood factors. Hepatocytes were isolated from pure Iberian (n=10) and Landrace (n=8) pigs of similar BW (24.5±12.1 and 32.9±6.1 kg BW, respectively), by collagenase perfusion. Monolayers were established in medium containing fetal bovine serum for 1 day and switched to serum-free medium for the remainder of the culture period. Hepatocytes were maintained in William’s E supplemented with β-mercaptoethanol (0.1 mM), glutamine (2 mM), antibiotics (gentamicin, penicillin, streptomycin and amphotericin B), dimethyl sulfoxide (1 µg/ml), dexamethasone (10−8 M), insulin (0.173 and 17.3 nM) and glucagon (0.287, 2.87 and 28.7 nM) for 24 to 48 h. Gluconeogenesis (GNG), glycogen degradation, triglycerides (TG) content and esterification, β-hydroxybutyrate (BHB) synthesis, IGF-1 synthesis, albumin and urea synthesis were determined. Iberian pigs had greater capacity of GNG than Landrace (24%, P<0.05), although no difference in glycogen degradation was found (P>0.10). TG content and esterification tended to be lower in hepatocytes from Iberian compared with Landrace pigs (12% and 31%, respectively; 0.10<P<0.05). Furthermore, addition of free fatty acids (CLA or linoleic acid, 0.2 mM) increased TG content (64%, P<0.001) although no difference between fatty acids was found. When free fatty acids were compared, a trend toward increased esterification (41%, P=0.078) was found for CLA. Although glucagon stimulated and insulin inhibited BHB synthesis, no difference between breeds was found (P>0.10). IGF-1 synthesis was diminished in hepatocytes from Iberian compared with Landrace pigs (16%, P<0.05). On the contrary, rate of albumin synthesis was greater in Iberian compared with Landrace pigs (58%, P<0.05). Finally, the capacity of urea synthesis was lower in hepatocytes of Iberian compared with Landrace pigs (37%, P<0.05). When ammonia was added to the media, urea concentration increased (648%, 1108% and 2791% when 0 mM was compared with 2.5, 5 and 10 mM, respectively). Urea synthesis increased on increasing ammonia content (55% and 325% when 0 mM was compared with 5 and 10 mM, respectively; P<0.0001). In conclusion, the genetic background accounts for important differences in protein and energy metabolism pathways found in primary culture of hepatocytes from lean and obese pigs.

According to present acts and regulations, farmed foxes shall have a gnawing or other enrichment object in their cages. However, research on the welfare effects of gnawing objects has been scarce. We assessed physiology and health, that is weight development, urinary cortisol-creatinine ratio, serum cortisol level after adrenocorticotropic hormone administration, internal organ masses and incidence of gastric ulcerations as well as dental and overall oral health, in pair-housed juvenile blue foxes that were housed either with or without a possibility to interact with bones (cattle femur) during their growing season (July to December). The results show that the physiological effects of the possibility to interact with bones were either non-significant or suggested that competition for bones may jeopardize the welfare of subordinate individuals. However, the results clearly show that gnawing bones are beneficial for the dental health of farmed foxes.

Colonization of the gastrointestinal tract of cattle by Shiga toxin-producing Escherichia coli increases the risk of contamination of food products at slaughter. Our study aimed to shed more light on the mechanisms used by E. coli O157:H7 to thrive and compete with other bacteria in the gastrointestinal tract of cattle. We evaluated, in vitro, bovine intestinal mucus and its constituents in terms of their capacity to support growth of E. coli O157:H7 in presence or absence of fecal inoculum, with and without various enzymes. Growth of E. coli O157:H7 and total anaerobic bacteria were proportionate to the amount of mucus added as substrate. Growth of E. coli O157:H7 was similar for small and large intestinal mucus as substrate, and was partially inhibited with addition of fecal inoculum to cultures, presumably due to competition from other organisms. Whole mucus stimulated growth to the greatest degree compared with other compounds evaluated, but the pathogen was capable of utilizing all substrates to some extent. Addition of enzymes to cultures failed to impact growth of E. coli O157:H7 except for neuraminidase, which resulted in greater growth of E. coli O157 when combined with sialic acid as substrate. In conclusion, E. coli O157 has capacity to utilize small or large intestinal mucus, and growth is greatest with whole mucus compared with individual mucus components. There are two possible explanations for these findings (i) multiple substrates are needed to optimize growth, or alternatively, (ii) a component of mucus not evaluated in this experiment is a key ingredient for optimal growth of E. coli O157:H7.

Published studies have shown that methane yield (g CH4/kg dry matter) from sheep is positively correlated with the size (volume and surface area) of the reticulo-rumen (RR) and the weight of its contents. However, the relationship between CH4 yield and RR shape has not been investigated. In this work, shape analysis has been performed on a data set of computerised tomography (CT) scans of the RR from sheep having high and low CH4 yields (n=20 and n=17, respectively). The three-dimensional geometries of the RRs were reconstructed from segmented scan data and split into three anatomical regions. An iterative fitting technique combining radial basis functions and principal component (PC) fitting was used to create a set of consistent landmarks which were then used as variables in a PC analysis to identify shape variation within the data. Significant size differences were detected for regions corresponding to the dorsal and ventral compartments between sheep with high and low CH4 yields. When the analysis was repeated after scaling the geometries to remove the effect of size, there was no significant shape variation correlating with CH4 yield. The results have demonstrated the feasibility of CT-based computational shape determination for studying the morphological characteristics of the RR and indicate that size, but not shape correlates with CH4 yield in sheep.

The European Union Effort Sharing Regulation (ESR) will require a 30% reduction in greenhouse gas (GHG) emissions by 2030 compared with 2005 from the sectors not included in the European Emissions Trading Scheme, including agriculture. This will require the estimation of current and future emissions from agriculture, including dairy cattle production systems. Using a farm-scale model as part of a Tier 3 method for farm to national scales provides a more holistic and informative approach than IPCC (2006) Tier 2 but requires independent quality control. Comparing the results of using models to simulate a range of scenarios that explore an appropriate range of biophysical and management situations can support this process by providing a framework for placing model results in context. To assess the variation between models and the process of understanding differences, estimates of GHG emissions from four farm-scale models (DairyWise, FarmAC, HolosNor and SFARMMOD) were calculated for eight dairy farming scenarios within a factorial design consisting of two climates (cool/dry and warm/wet)×two soil types (sandy and clayey)×two feeding systems (grass only and grass/maize). The milk yield per cow, follower:cow ratio, manure management system, nitrogen (N) fertilisation and land area were standardised for all scenarios in order to associate the differences in the results with the model structure and function. Potential yield and application of available N in fertiliser and manure were specified separately for grass and maize. Significant differences between models were found in GHG emissions at the farm-scale and for most contributory sources, although there was no difference in the ranking of source magnitudes. The farm-scale GHG emissions, averaged over the four models, was 10.6 t carbon dioxide equivalents (CO2e)/ha per year, with a range of 1.9 t CO2e/ha per year. Even though key production characteristics were specified in the scenarios, there were still significant differences between models in the annual milk production per ha and the amounts of N fertiliser and concentrate feed imported. This was because the models differed in their description of biophysical responses and feedback mechanisms, and in the extent to which management functions were internalised. We conclude that comparing the results of different farm-scale models when applied to a range of scenarios would build confidence in their use in achieving ESR targets, justifying further investment in the development of a wider range of scenarios and software tools.

Due to genetic selection for fast growth and high breast meat yield, commercial strains of broiler chickens and broiler breeders are predisposed to high feed intake; however, feeding broiler breeders ad libitum impairs their health and reproductive performance. Broiler breeders are feed-restricted throughout rearing to maintain health and performance, yet feed restriction results in hunger, feeding frustration and lack of satiety. The objective of this study was to examine the effect of alternative feeding strategies, including feed additives (separately or combined) and a fixed non-daily feeding schedule, on the feeding motivation and welfare of broiler breeders during rearing. At 3 weeks of age, 180 Ross 308 breeder pullets were allocated to 90 cages and fed with one of five isocaloric treatments: (1) daily control diet (control), (2) daily calcium propionate diet (CaP), (3) daily soybean hull diet (SBH), (4) daily alternative diet (alternative: CaP + SBH) and (5) 4/3 control diet (four on-feed days and three non-consecutive off-feed days per week). The CaP diet included calcium propionate at 1.4% from 3 to 6 weeks of age, and at 3.2% from 7 to 12 weeks of age, and the SBH diet contained soybean hulls included at 40%. The alternative diet included both soybean hulls and calcium propionate at the same inclusion rate as the SBH and CaP diets, respectively. Pullets were weighed and scored for feather coverage every week. A feed intake test was conducted at 3, 4, 8, 10 and 11 weeks of age for 10 min during on- and off-feed days. At 12 weeks of age, feather samples were analysed for fault bars. Data were analysed using linear mixed regression models, with cage nested in the models and age as a repeated measure. At 4 weeks of age, pullets fed soybean hull-enriched diets (SBH and alternative diets) and those on the 4/3 schedule had lower feed intake than control pullets (P = 0.02). Feathers from pullets fed the SBH diet had fewer fault bars than those fed the CaP diet (P = 0.04). The results indicated that the inclusion of soybean hulls (alone or combined with calcium propionate) and a 4/3 feeding schedule can reduce feeding motivation of broiler breeders during early rearing.

This study was conducted to investigate the pattern of plasma carotenoid concentration (PCC) in lambs switching from a low to a high dietary carotenoid level. A total of 12 Romane lambs were individually penned indoors and fed a low dietary carotenoid level for 23 days (period 1) and then a high dietary carotenoid level for 15 days (period 2). At the beginning of period 2, the lambs were 15 weeks old and weighed 29.9 kg (s.d. 2.7) on average. Lambs were fed daily (dry matter) 0.558 kg alfalfa pellets, 0.181 kg straw and 0.181 kg barley. Plasma content of total carotenoids was measured daily in period 2 by spectrophotometry. PCC (μg/l) varied with the animal (P < 0.001) and with time elapsed since the beginning of alfalfa distribution (P < 0.001). Mean PCC was 8 μg/l (s.d. 3.3) at the beginning of period 2, then increased curvilinearly with the time elapsed since the beginning of alfalfa distribution. As early as 24 h on the alfalfa diet, PCC was already higher than before the switch (P < 0.001). Mean PCC continued to increase until day 6 on average and reached a plateau thereafter. We propose a monomolecular function to model this pattern, the equation obtained on the mean data being: PCC = 97 (s.e. 2.2) × (1−exp(−0.3378 (s.e. 0.0282)×d)), where r2 = 0.997, r.s.d. = 4.47, n = 15 and d = day. The percentage of variance explained by the model ranged between 95.9% and 99.2%, depending on the animal. The parameters of the monomolecular function varied among animals, confirming the interindividual variability in animal response. Plateau for PCC was reached slightly earlier for the six lambs with the lowest values of the asymptote than for the six lambs with the highest values of the asymptote.

The low feed intake and stress associated with abrupt weaning in conventional pig farming often result in poor post-weaning performance, which is related to impaired intestinal function. We investigated effects of housing conditions before weaning on performance around weaning of relatively light and heavy piglets. Before weaning, piglets were housed either with five sows and their litters in a multi-suckling (MS) system or in pens with individually housed sows in farrowing crates (FC). After weaning at 4 weeks of age (day 0), 16 groups of four piglets (two light and two heavy litter-mates) were housed under equal conditions in enriched pens. Mannitol (day −5 and day 5) and galactose (day 5) were orally administered as markers for gastrointestinal carbohydrate absorption, and after 20 min a blood sample was taken (sugar absorption test). In addition, BW, feed intake and faecal consistency as an indicator for diarrhoea, were assessed frequently during 2 weeks post-weaning. Pre-weaning housing, weight class and their interaction did not affect post-weaning faecal consistency scores. Weight gain over 2 weeks did not differ between pre-weaning housing treatments, but MS piglets gained more (0.67±0.12 kg) than FC piglets (0.39±0.16 kg) between days 2 and 5 post-weaning, P=0.02), particularly in the ‘heavy’ weight class (interaction, P=0.04), whereas feed intake was similar for both treatments. This indicates a better utilisation of the ingested feed of the MS piglets compared with the FC piglets in the early post-weaning period. Pre-weaning mannitol concentrations were unaffected by pre-weaning housing, weight class and their interaction. On day 5 post-weaning, however, MS piglets had a lower plasma concentration of mannitol (320 v. 592 nmol/ml, SEM=132, P=0.04) and galactose (91 v. 157 nmol/ml, SEM=20, P=0.04) than FC piglets, regardless of weight class. In conclusion, MS and FC piglets differed in aspects of post-weaning gastrointestinal carbohydrate absorption and in weight gain between days 2 and 5 after weaning, but pre-weaning housing did not affect feed intake, weight gain and measures of faecal consistency over the first 2 weeks after weaning.

Silage cut twice annually (June and August) from a tetraploid red clover/grass sward and three times annually (May, July and September) from a low nitrogen (N) and high N perennial ryegrass/white clover sward was fed in proportion to dry-matter yield from each cut, over a 10-week period, each winter for 3 years to castrated male cattle of initial live weight 401 kg in year 1 and 425 kg in years 2 and 3. The silages were supplemented with 0, 1, 2 and 3 kg concentrate per head daily.

Total dry-matter yield from the red clover/grass sward was similar to that from the perennial ryegrass/white clover sward (high N grass) receiving 360 kg N per ha but the digestibility, particularly of first cut material was much lower. Dry-matter production of the low N grass/white clover sward was 0·73 of high N grass sward and produced silages of similar digestibility and fermentation.

Dry-matter intakes by the cattle were higher on the legume-based silages in years when clover made a worthwhile contribution to total yield, but this did not significantly improve utilization or animal performance compared with high N grass silage. Mean daily carcass gain per head on red clover/grass silage was 0·41 kg which was significantly less than the 0·61 kg on white clover/grass silage and 0·59 on high N grass (P < 0·001). Carcass output from red clover/grass silage was 618 kg/ha and 629 kg/ha from white clover/grass, both of which were significantly less than the 863 kg/ha from the high N grass silage (P < 0·001). Dressing proportion was also significantly poorer in animals fed red clover/grass silage compared with the other silage types.

There have been extensive studies in sheep and cattle considering cobalt (Co) supplementation and its effects on vitamin B12 concentrations in the body. However, there are limited studies on goats. The aim of this study was to compare two different sources of Co (sulfate v. glucoheptonate) at two different concentrations (0.25 and 0.5 mg/kg dry matter) in goat kid nutrition, and to evaluate the effects of these supplements on performance, serum vitamin B12, blood biochemistry and rumen volatile fatty acids. For this purpose, 30 weaned male goat kids were randomly allotted to five treatments. Serum vitamin B12 increased during the trial in the Co-supplemented groups. Co supplementation increased serum glucose concentrations. On day 35, Co-supplemented groups had greater glucose concentrations compared with control. Propionic+iso-butyric acid concentrations increased only in the 0.5 mg Co glucoheptonate treatment (P<0.05). Our results suggest that, despite the two sources of Co proving mostly similar, the main advantage of Co glucoheptonate compared with Co sulfate was in the ruminal synthesis of vitamin B12. However, although providing Co at National Research Council recommendation levels maintained vitamin B12 above or at normal concentrations, Co supplementation of the Co sufficient basal diet increased vitamin B12 and glucose concentrations.

1. Weaned single-suckled calves (castrates) were housed at 260·7 kg live weight. Six genotypes, all two- or three-way crossbreds, were represented. A diet of bruised barley, wet distiller's grains, swedes and grass silage was offered until the required minimum live weight (363 kg) and body composition was reached. The cattle were then slaughtered (treatment A), or retained for 8 (treatment B) or 16 weeks (treatment C). During the period when treatment B and C animals were retained their diet was 53% bruised barley and 47% wet distiller's grains offered ad libitum.

2. Live weights at slaughter were 418·2, 458·6 and 496·1 kg (P < 0·001), carcass weights 240·5, 266·2 and 295·7 kg (P < 0·001), and killing-out percentages 57·5, 58·1 and 59·6 (P<0·01) for treatments A, B and C respectively. Daily live-weight gain did not decline during the 8 or 16 weeks cattle were retained on treatments B and C.

3. Retaining the cattle to slaughter points B and C caused increases in the percentage of low-value joints in the carcass, carcass fat percentage, dry matter and fat contents of the dissected m. longissimus dorsi; and decreases in the percentage of high-value joints, carcass lean and bone percentage, crude protein and ash contents of m. longissimus dorsi. The carcass composition was 55·6, 53·5 and 51·4% lean (P<0·001); 30·7, 33·3 and 36·2% fat (P<0·001); 13·7, 13·2 and 12·4% bone (P< 0·001) for treatments A, B and C respectively.

4. The results are discussed in relation to the choice made by beef producers between slaughter and retention of cattle which have reached suitable live weight and condition for marketing.

Five years' records of unculled White Wyandotte flocks at Hillsborough, Northern Ireland, were analysed to give estimates of heritabilities and of genetic and environmental correlations by two methods. Pooling data for four years and eliminating hatch effects gave heritabilities of 0·28 for eggs laid by survivors to 500 days; 0·61 for weight of pullets at penning; 0·51 for weight of pullets at 8 weeks; 0·18 for days to first egg; and 0·71 for spring egg weight. These are in general agreement with most other published figures, as are most of the genetic correlations. A suggestion of sex-linkage in the genetic correlations between weight at 8 weeks and days to maturity was not supported by other figures from the data. The method of eliminating hatch effects, unexpectedly, gave higher heritability figures where heritability was already moderate or high. It gave numerically greater figures in most genetic correlations, but did not lead to an expected reduction in the environmental correlations. The data were probably inadequate for a rigorous test of the method.

A numerical model of male and female replacement processes is developed and used to construct a matrix which specifies the numbers of lactations of different parities expressed in a given time period by daughters of bulls used in artificial insemination (AI).

Populations resulting from newly established AI breeding programmes contain an unduly high proportion of offspring by young bulls. Subsequently an equilibrium is reached in which offspring of young and tested bulls are present in proportions determined by the relative usage of the two types of sire and by differences in replacement rates amongst their progeny. Once an equilibrium has been reached the numbers of progeny sired by bulls lacking a progeny test proof is extremely small.

The use of the technique to solve a number of specific planning problems is demonstrated.

Since, under Irish conditions, the source of protein used in pig rations often varies from one area to another, an experiment was conducted to determine the accumulation of copper in the tissues of pigs given the commonly-used

protein sources in diets containing 250 ppm of supplemental copper. The protein sources compared were (a) white-fish meal (370 ppm Cu), (b) soya bean meal (17 ppm. Cu), (c) roller-dried skim milk (2 ppm Cu), and (d) a mixture of soya bean meal and meat and bone meal (5 ppm Cu) (Table 1). The minerals, vitamins and copper sulphate were premixed with about 5 kg. of barley and distributed over the total feed before mixing. The copper sulphate was sieved to remove coarse particles. Analyses of complete feed mixtures are shown in Table 1. As far as possible, the rations were balanced for crude protein, calcium and phosphorus.

Experimental designs with optimal allocation of animals within and between breeds are given for investigating inter-breed relationships between different traits. Optimal designs are given for estimating the following between-breed parameters: the genetic regression with breeds random, the genetic correlation, the ratio of genetic standard deviations for two traits and also the comparison of regressions within and between breeds. As in the case of between-breed variation, these turn out to be multibreed designs with a few animals per breed and usually as many different breeds as possible. Formulae are given for obtaining optimal numbers and estimation errors for traits with intra- and inter-breed correlations lying within restricted ranges.

If an exepriment with a multibreed design is to be effective, the optimum number of animals (or sires) per breed would normally lie between two and 10. If the optimum number per breed is much greater than 10, the experiment is unlikely to be worthwhile unless the total number of animals is large (at least several thousand).

Finn Dorset ewes were offered forage ad libitum from week 16 of pregnancy until lambing and were either unsupplemented (U) or given 600 g/day of concentrates (S). Grass silage and white clover silage, ensiled separately and having similar dry matter concentrations, were offered in four mixtures with white clover proportions of 0, 0·20, 0·40 and 0·60 on a fresh basis. Forage intake was significantly higher for mixtures containing higher proportions of clover (9·1, 10·5, 12·3 and 13·6 g organic matter (OM) per kg live weight) and the ewes gained more weight (33, 65, 178 and 174 g/day) and had smaller losses in body condition score (–0·59, –0·49, –0·39 and –0·17). Higher proportions of clover in the diet in late pregnancy also resulted in significantly higher growth rates of lambs during lactation, when a common diet was offered.

The number of foetuses carried in pregnancy had significant effects on intake and some aspects of performance. For ewes carrying singles, twins and multiples respectively, mean daily forage intakes were 12·2, 11·9 and 10·4 g OM per kg live weight and losses in body condition score were –0·05, –0·40 and –0·62.

Offering the supplement reduced forage intake and for treatments U and S respectively, mean daily values were 12·6 and 10·2 g OM per kg live weight. Whilst the supplemented ewes had smaller losses in body condition score in late pregnancy (–0·54 v. –0·28), lamb birth weights and growth rates were significantly increased only for ewes which had carried three or more foetuses in pregnancy and reared their two heaviest lambs.

The effect of pregnancy on the fat and protein contents of milk was studied on a 414-lactation sample. One hundred and forty-nine lactations of non-pregnant cows managed under identical conditions served as controls. The difference between individual fat or protein contents of each pregnant animal and the mean corresponding values in controls was computed weekly from the week of conception. The effect of pregnancy on fat and protein concentrations began to be significant from the 20th week of pregnancy, regardless of the week of conception. It was higher in the Friesian breed than in Holstein or Montbeliarde cows. In Holstein and Montbeliarde cows, the effect of pregnancy was higher in high producing multiparous cows (+2·8 and +2·9 g/kg for fat and protein concentrations, respectively, at the 29th pregnancy week) than in primiparous or low producing multiparous cows (+2·0 and +1·4 g/kg, for fat and protein concentration, respectively). In each of these three lactation groups (Friesian, high producing multiparous Holstein and Montbeliarde cows, other Holstein and Montbeliarde cows) the following linear model

was fitted, where Y is the difference between fat or protein concentration of pregnant and non pregnant cows at a given stage (week), Pw is the pregnancy week, and a and b are parameters. This increase in milk fat and protein concentrations in late pregnancy cannot compensate the concomitant decrease in milk yield, so that fat and protein yield decreased in the same manner as milk yield during pregnancy (-77 glday and -68 glday at the 29th week of pregnancy, for fat and protein yield, respectively).

Nineteen field trials were conducted in eight 1 uropean countries to examine the effects of tylosin, administered in the food at a level of 20mg/kg to healthy pigs under good management conditions. Average daily gain and food conversion ratio were improved by 33 g and 0·17 respectively over untreated control animals, with no change in average daily food consumption. These improvements in growth performance were similar in the grower and finisher phases, and the response to tylosin was not affected by sex, initial weight or year. Absolute responses in average daily gain and efficiency of food utilization were constant, whatever the level of performance in the controls.

Early-cut, precision-chopped (EP) and late-cut, flail-harvested (LF) silages were offered ad libitum to breeding ewes during mid and late pregnancy in two experiments. Silages offered in late pregnancy were factorially arranged with silages offered in mid pregnancy giving four silage treatments. Voluntary drymatter intake, ewe weight change, lamb birth weight and blood levels of 3-hydroxybutyrate (3-OHB) and non-esterifiable free fatty acids (NEFA) were measured. The intake of LF silage was proportionately 0·61 and 0·72 of that of EP silage in mid and late pregnancy respectively. While type of silage given in mid pregnancy did not affect the mean silage intake in weeks 15 to 20 of pregnancy, the rate of decline in intake was higher and the intake during week 20 of pregnancy was lower following the feeding of EP silage in mid pregnancy. Despite a considerably lower level of body reserves associated with LF silage given in mid pregnancy, there was little effect on levels of 3-OHB or NEFA in late pregnancy or on lamb birth weight.

Dairy operations located in many temperate climates experience a decline in milk production associated with short-term moderate heat stress during the summer months. The duration and intensity of this heat stress differs dramatically from the sustained, intense heat experienced in many tropical and subtropical countries where the vast majority of heat stress research has been conducted. As such, many of the strategies utilized to ameliorate production losses associated with sustained, intense heat may not be effective in temperate climates. The objective of this research was to characterize the production responses of lactating dairy cows during and after short-term, moderate heat exposure and, to determine if feeding a fungal culture, Aspergillus oryzae, during a 5-day heat stress period, could effectively alleviate the associated production losses. In a two-period, cross-over design, eight mature lactating cows were given a total mixed ration with or without Aspergillus oryzae. Each 15-day period consisted of a 5-day thermoneutral phase, a 5-day heat stress phase and a 5-day thermoneutral recovery phase. When exposed to moderate heat stress for a 5-day period, cows experienced a rise in vaginal temperature and a decline in dry-matter intake. Following the 5-day heat stress phase, milk yield declined by a factor of 0·09. Supplementation with Aspergillus oryzae had no effect on vaginal temperature, dry-matter intake, water intake, milk yield or milk components. These data indicate that short-term, moderate heat stress, which occurs during the spring and summer months in temperate climates, will significantly decrease production in the lactating cow. Addition of fungal cultures to the diet during the period of imposed heat did not ameliorate production losses associated with this type of heat stress.