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Portal vein, hepatic vein and circulating plasma amino acids in rats given diets based on lactalbumin, faba bean (Vicia faba) and chickpea (Cicer arietinum)

Published online by Cambridge University Press:  18 August 2016

L. A. Rubiot
L. A. Rubiot*
Affiliation:
Estación Experimental del Zaidín, Unidad de Nutrición, Profesor Albareda, 1, 18008 Granada, Spain
*
†E-mail:[email protected]
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Abstract

Three experiments were carried out to determine plasma amino acids concentrations in circulating, portal and hepatic blood of growing male Wistar rats given diets containing lactalbumin, faba beans or chickpeas as the only protein source. Diets contained the same amount of digestible energy (15·5 kJ/g) and protein (lactalbumin in controls or legume proteins in the experimental diets; 100 g/kg). Appropriate amounts of essential synthetic amino acids were also added to legume-based diets taking into account their amino acid composition to equalize them to control (lactalbumin) diets. Portal blood flow (8·7±0·3 ml/min) was measured by using a transit-time ultrasound flow probe. Higher (P < 001) plasma concentrations of methionine than of controls were determined in hepatic veins of legume-fed rats. In contrast, lower (P < 001) concentrations of threonine, proline, valine, leucine, phenylalanine and lysine than those of controls were found in faba bean- and chickpea-fed rats. The same result as hepatic was obtained for portal and circulating plasma samples except that alanine and histidine values of legume-fed rats were also lower (P < 001) than controls. Calculated net afferent appearance rates of amino acids to the liver were lower (P < 001) than controls in rats given faba bean and chickpea diets for threonine, alanine, proline, valine, leucine, phenylalanine and lysine. This lower contribution of amino acids to the liver mainly via the portal vein in faba bean or chickpea-fed rats might explain previously reported differences in protein utilization and growth in comparison with animals given other protein sources (lactalbumin).

Keywords

amino acidsCicer arietinumlactalbuminratsVicia faba
Type
Growth, development and meat science
Information
Animal Science , Volume 77 , Issue 1 , August 2003 , pp. 3 - 10
Copyright
Copyright © British Society of Animal Science 2003

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