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The effect of basal diet on lactate-producing bacteria and the susceptibility of sheep to lactic acidosis

Published online by Cambridge University Press:  18 August 2016

R. A. M. Al Jassim ,
G. L. R. Gordon  and
J. B. Rowe
R. A. M. Al Jassim
Affiliation:
Division of Animal Science, University of New England, Armidale, New South Wales 2351, Australia
G. L. R. Gordon
Affiliation:
Division of Animal Science, University of New England, Armidale, New South Wales 2351, Australia
J. B. Rowe
Affiliation:
Division of Animal Science, University of New England, Armidale, New South Wales 2351, Australia
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Abstract

The influence of a diet of either pasture or hay on the development of lactic acidosis in sheep was investigated using a grain challenge approach. Twenty-four Merino wethers with a mean live weight of 36·7 (s.e.3·6) kg were used; 12 were adapted to grass pasture and 12 to hay (lucerne and oaten hay, 60: 40) for 4 weeks before being given 1 kg of crushed barley via stomach tube. Six sheep in each group were also given virginiamycin (VM; 50 mg/kg barley) with the grain to test the efficacy of this antibiotic in controlling the bacteria responsible for the development of acidosis. Changes in volatile fatty acid (VFA), pH, lactate and bacterial count in the rumen and faecal pH and dry matter (DM) were measured for a 24-h period following administration of the barley. Daily intakes of hay were measured for a 10-day period following grain engorgement. Total ruminal VFA increased (P < 0·01) over time and tended (P = 0·08) to be higher in sheep adapted to hay than in those adapted to pasture (67·5 v. 59·8 mmol/l). The molar proportions of VFA changed (P < 0·01) over time in favour of propionate in both groups. Ruminal pH was higher (P < 0·001) in pasture-adapted sheep, but declined (P < 0·001) in both groups over time following the introduction of barley. This decline in pH was associated with increases in ruminal concentration of VFA in pasture-adapted sheep and VFA and lactate in hay-adapted sheep. The addition of VM resulted in a higher (P < 0·001) proportion of propionate and a trend towards higher (P = 0·24) faecal pH and DM content. Faecal pH and DM content declined (P < 0·001) over time and was lower for the pasture-adapted sheep. The introduction of either barley alone or barley with VM from both hay and pasture diets increased (P < 0·05) the viable counts of total bacteria, Streptococcus bovis and lactic acid bacteria. Bacterial isolates were purified and identified by complete sequencing of the 16S rRNA gene to determine the predominant bacteria during the overfeeding of grain. Isolates from medium selective for S. bovis were all identified as this species when VM was not given. VM had no effect on counts of viable bacteria, but inhibited the growth of S. bovis.

This study has shown that sheep given hay are more susceptible to lactic acidosis, the signs of which can be reduced by VM.

Keywords

acidosisfeed grainslactic acid bacteriaStreptococcus bovisvirginiamycin
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 77 , Issue 3 , December 2003 , pp. 459 - 469
Copyright
Copyright © British Society of Animal Science 2003

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References

Al Jassim, R. A. M. and Rowe, J. B. 1999. Better understanding of acidosis and its control. Recent Advances in Animal Nutrition in Australia 12: 91–97.Google Scholar
Allison, M. J., Robinson, I. M., Dougherty, R. W. and Bucklin, J. A. 1975. Grain overload in cattle and sheep: changes in microbial population in the cecum and rumen. American Journal of Veterinary Research 36: 181–185.Google ScholarPubMed
Caldwell, D. R. and Bryant, M. P. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied Microbiology 14: 794–801.CrossRefGoogle ScholarPubMed
Chaucheyras, F., Fonty, G., Bertin, G., Salmon, J. and Gouet, P. 1996. Effect of a strain of Saccharomyces cerevisiae (Levucell® SC), a microbial additive for ruminants, on lactate metabolism in vitro. Canadian Journal of Microbiology 42: 927–933.CrossRefGoogle Scholar
Courtney, D. A. and Seirer, R. C. 1996. Supplementary feeding of grain to cattle with virginiamycin to reduce the risk of acidosis. Proceedings of the Australian Society for Animal Production 21: 344 (abstr. ).Google Scholar
De Man, J. C., Rogosa, M. and Sharp, M. E. 1960. A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23: 130–135.CrossRefGoogle Scholar
Dunn, B. H., Emerick, R. J. and Embry, B. 1979. Sodium bentonite and sodium bicarbonate in high-concentrate diets for lambs and steers. Journal of Animal Science 48: 764–769.CrossRefGoogle Scholar
Goad, D. W., Goad, C. L. and Nagaraja, T. G. 1998. Ruminal microbial and fermentative changes associated with experimentally induced sub-clinical acidosis in steers. Journal of Animal Science 76: 234–241.CrossRefGoogle Scholar
Godfrey, S. I., Rowe, J. B., Speijers, E. J. and Toon, W. 1993. Lupin, barley, or barley plus virginiamycin as supplement for sheep at different feeding intervals. Australian Journal of Experimental Agriculture 33: 135–140.CrossRefGoogle Scholar
Godfrey, S. I., Rowe, J. B., Thorniley, G. R., Boyce, G. R. and Speijers, E. J. 1995. Virginiamycin to protect sheep fed wheat, barley or oats from grain poisoning under simulated drought feeding conditions. Australian Journal of Agricultural Research 46: 393–401.CrossRefGoogle Scholar
Gottschall, D. W., Gombatz, C. and Wang, R. 1987. Analysis of tissue residues and comparative metabolism of virginiamycin in rats, turkeys and cattle. Journal of Agricultural and Food Chemistry 35: 900–904.CrossRefGoogle Scholar
Hungate, R. E. 1969. A roll tube method for cultivation of strict anaerobes. In Methods in microbiology, vol. 3 (ed. Norris, J. R. and Ribbons, E. W.), pp. 117–132. Academic Press, New York.Google Scholar
Huntington, G. B. 1993. Nutritional problems related to the gastro-intestinal tract: acidosis. In The ruminant animal: digestive physiology and nutrition (ed. Church, D. C.), pp. 474–480. Waveland Press Inc., IL.Google Scholar
Huntington, G. B., Emerick, R. J. and Embry, L. B. 1977. Sodium bentonite effects when fed at various levels with high concentrate diets to lambs. Journal of Animal Science 45: 119–125.CrossRefGoogle Scholar
Kezar, W. W. and Church, D. C. 1979. Effect of thiopeptin and bicarbonate on the prevention of lactic acidosis induced in sheep. Journal of Animal Science 49: 1396–1402.CrossRefGoogle ScholarPubMed
Kung, L. and Hession, A. O. 1995. Preventing in vitro lactate accumulation in ruminal fermentation by inoculation with Megasphaera elsdenii . Journal of Animal Science 73: 250–256.CrossRefGoogle ScholarPubMed
Lane, D. J. 1991. 16S rRNA sequencing. In Nucleic acid techniques in bacterial systematics (ed. Stackebrandt, E. and Goodfellow, M.), pp. 115–175. Academic Press, Chichester.Google Scholar
Mackie, R. I., Gilchrist, F. M. C., Robberts, A. M., Hannah, P. E. and Schwartz, H. M. 1978. Microbiological and chemical changes in the rumen during the stepwise adaptation of sheep to high concentrate diets. Journal of Agricultural Science, Cambridge 90: 241–252.CrossRefGoogle Scholar
Ministry of Agriculture, Fisheries and Food. 1981. The analysis of agricultural materials, second edition. A manual of the analytical methods used by the Agricultural Development and Advisory Service, pp. 1–6.Google Scholar
Nagaraja, T. G., Avery, T. B., Bartley, E. E., Galitzer, S. J. and Dayton, A. D. 1981. Prevention of lactic acidosis in cattle by lasalocid or monensin. Journal of Animal Science 53: 206–216.CrossRefGoogle ScholarPubMed
Nagaraja, T. G., Avery, T. B., Galitzer, S. J. and Harmon, D. L. 1985. Effect of ionophore antibiotics on experimentally induced lactic acidosis in cattle. American Journal of Veterinary Research 46: 2444–2452.Google ScholarPubMed
Nagaraja, T. G., Godfrey, S. I., Winslow, S. W. and Rowe, J. B. 1995. Responses in ciliated protozoa and rumen fermentation in sheep with barley plus virginiamycin. Australian Journal of Agricultural Research 46: 1137–1147.CrossRefGoogle Scholar
Oragui, J. I. and Mara, D. D. 1984. A note on a modified membrane-Bovis agar for the enumeration of Streptococcus bovis by membrane filtration. Journal of Applied Bacteriology 56: 179–181.CrossRefGoogle ScholarPubMed
Osborne, D. R. and Voogt, P. 1978. The analysis of nutrient in foods. Academic Press, London.Google Scholar
Owens, F. N., Secrist, D. S., Hill, W. J. and Gill, D. R. 1998. Acidosis in cattle: a review. Journal of Animal Science 76: 275–286.CrossRefGoogle ScholarPubMed
Rogers, J. A., Branine, M. E., Miller, C. R., Wray, M. I., Bartle, S. J., Preston, R. L., Gill, D. R., Pritchard, R. H., Stilborn, R. P. and Bechtol, D. T. 1995. Effects of dietary virginiamycin on performance and liver abscess incidence in feedlot cattle. Journal of Animal Science 73: 9–20.CrossRefGoogle ScholarPubMed
Schwartz, H. M. and Gilchrist, F. M. C. 1974. Microbial interactions with the diet and the host animal. In Digestion and metabolism in the ruminant (ed. Mc, I. W.Donald and A. Warner, C. I.), pp. 165–179. University of New England Publishing Unit, Armidale, Australia.Google Scholar
Statgraphics Plus. 1996. Using general linear regression models (GLM). Manugistics Inc., Rockville, MD.Google Scholar
Thorniley, G. R., Rowe, J. B., Cowcher, P. C. and Boyce, M. D. 1998. A single drench of virginiamycin to increase safety of feeding grain to sheep. Australian Journal of Agricultural Research 49: 899–906.CrossRefGoogle Scholar
Wallace, R. J. 1996. Rumen microbiology and efficiency of digestion. In Milk composition, production and biotechnology (ed. Welch, R. A. S., Burns, D. J. W., Davis, S. R., Popay, A.I. and Prosser, C. G.), pp. 465–487. CAB International, Wallingford.Google Scholar
Wells, J. E., Krause, D. O., Callaway, T. R. and Russell, J. B. 1997. A bacteriocin-mediated antagonism by ruminal lactobacilli against Streptococcus bovis . FEMS Microbiology Ecology 22: 237–243.CrossRefGoogle Scholar
Wiryawan, K. G. and Brooker, J. D. 1995. Probiotic control of lactate accumulation in acutely grain-fed sheep. Australian Journal of Agricultural Research 46: 1555–1568.CrossRefGoogle Scholar