The Preble's meadow jumping mouse (Zapus hudsonius preblei) is listed as a threatened subspecies under the United States Endangered Species Act (US-ESA). The quantitative description of this subspecies was based on cranial measurements of only three adult specimens. It is one of twelve subspecies of Z. hudsonius and is a peripheral population at the western edge of its range. We tested the uniqueness of Z. h. preblei relative to other nearby subspecies of Z. hudsonius using a hypothesis testing approach and analyses of cranial morphometric, mtDNA sequence and nuclear microsatellite data obtained from museum specimens and archived tissues. Morphometric analysis of variance did not support the original description of Z. h. preblei as a subspecies. Principal component analysis of these data showed Z. h. preblei within the range of variation found in Z. h. campestris and Z. h. intermedius. Discriminant analysis correctly classified only 42% of Z. h. preblei skulls at jackknifed posterior probabilities >0.95 relative to Z. h. campestris. All mtDNA haplotypes found in Z. h. preblei were also found in Z. h. campestris. Simulation based estimates of current and historical gene flow (MDIV) revealed low, but non-zero, mtDNA gene flow among Z. h. preblei and several nearby subspecies. Analyses of five nuclear microsatellite loci using population pairwise FST, BAPS and STRUCTURE were consistent with morphometric and mtDNA results. These revealed low levels of genetic structure and evidence of recent gene flow and bottlenecks in Z. h. preblei. Due to a lack of clearly recognisable genetic, morphological, or adaptive differences, we synonymise Z. h. preblei and Z. h. intermedius with Z. h. campestris. We suggest that candidates for listing under the US-ESA, or similar biodiversity laws, be evaluated for genetic and/or morphological uniqueness to prevent the misallocation of resources to non-distinct taxa like Z. h. preblei.

Despite the alarming rate of tropical deforestation, the long-term conservation value of forest fragments remains poorly understood. We report on the avifaunal turnover in an isolated 4 ha tropical forest fragment in Singapore (i.e. Singapore Botanic Gardens rainforest fragment (SBGRF)) between 1898 and 1998. Over 100 years, the SBGRF lost 18 (49%) species and gained 20 species. More forest-dependent species (3) were lost from the SBGRF than survived (1) or colonised it (no species). Conversely, significantly more introduced species (4) colonised the fragment than were previously recorded (1 species). Significantly more nectarivores survived (8 species) or colonised (9 species) than were lost (two species). In essence, while the avian species richness in the SBGRF remained relatively constant after a century, its species composition underwent significant changes. The avian species composition in the SBGRF in 1998 appeared to be more similar to that of the contemporary smaller and younger Singaporean secondary forest patches than to either the larger and older forest reserves or to the SBGRF 100 years ago. Our study suggests that small isolated tropical forest fragments may have limited long-term conservation value for native forest bird species.

Many diurnal raptors are vulnerable to modern forestry and areas surrounding individual raptor nests are often strictly protected. This study compared random forest plots and nest sites of five species of forest-dwelling raptors in Estonia to explore whether timber harvesting per se reduces the probability of raptor nesting when controlling for the effect of nest tree availability and stand structure. Medium-sized species – the honey buzzard (Pernis apivorus), goshawk (Accipiter gentilis), common buzzard (Buteo buteo) and lesser spotted eagle (Aquila pomarina) – preferred natural to managed forests but the preference, when controlling for other stand characteristics, was not significantly related to the incidence of cutting. Instead, the presence of nests depended on structural features of old growth, even for the sparrowhawk (Accipiter nisus), which preferred young secondary forests. The sparrowhawk was the only species for which thinning had an independently negative influence on nest presence. The results stress the importance of structural diversity of forest stands in multiple-use forestry and indicate that if natural stand structure is preserved and disturbance avoided in buffer zones around the nests of medium-sized raptors, some non-commercial cutting (firewood collecting, sanitary cutting) may be allowed there outside the breeding season.

Large carnivores have declined worldwide, largely through conflict with people. Here, we quantify the impact of lethal control, associated with livestock depredation, on a population of African lions (Panthera leo) living outside protected areas. Farmers shot lions only in response to livestock attacks. Nevertheless, adult mortality was high and a simple model predicted that the population was marginally stable or slowly declining. Mortality was four times higher among lions radio-collared in association with attacks on livestock, than among lions with no known history of stock killing, suggesting that some animals were habitual stock killers. Known stock killers also experienced lower reproductive success; hence there was strong artificial selection against stock-killing behaviour. In addition, mortality was higher among lions whose home ranges overlapped a property where non-traditional livestock husbandry was associated with chronic depredation by lions. This 180 km2 ranch acted as a sink that directly affected lions over more than 2000 km2 and may have undermined the viability of the study population. Our results suggest that sustainable coexistence of lions and people demands livestock husbandry that effectively deters predators from acquiring stock-killing behaviour, but that lethal control may play an important role in avoiding the spread of such behaviours through the population.

In Asia, subtropical tall grasslands and their associated fauna are mostly confined to the floodplains of the large rivers draining the Himalayan range. In recent decades, cultivation and hydroelectric dam construction have severely reduced their spatial abundance. During 1998–2000, we conducted a detailed study of the habitat use of hog deer, Axis porcinus, an important prey species of tiger, Panthera tigris, in the floodplains of the large Karnali river in Royal Bardia National Park, Nepal. Strong preference for tall grass and avoidance of the successional stages following riparian forests throughout the year was revealed by analyses of tracking data from 18 radio-collared deer and from density estimates from block counts (n=46) in different habitat types. Continuously strong selection for tall grass despite the reduced availability of this habitat type during the monsoonal flooding indicated an insignificant effect of density-dependent factors in habitat selection. Reduced availability of cover following traditional cutting and burning of the tall grasslands by local villagers did not produce changes in habitat use or an increase in daily movements, indicating an absence of trade-offs between habitat preference for food and for predator avoidance. By regulating fluvial action, hydroelectric power development will lead to the loss of tall grasslands due to accelerated secondary succession, which will therefore adversely affect the viability of local populations.

The black and white ruffed lemur, Varecia variegata, which is endemic to Madagascar, is under the threat of extinction due to the loss and fragmentation of its natural habitat, a consequence of deforestation. Behavioural researchers studying a population of V. variegata inhabiting the Manombo Special Reserve, noted that over a 4-year period, none of the family groups within the reserve produced offspring while those in neighbouring populations had been reproductively successful. Inbreeding depression was postulated as the trigger for this occurrence. However, another explanation exists. A cyclone, striking the region in 1997, destroyed a large percentage of the mature fruit-producing trees that V. variegata relied upon as a food source, which is likely to have produced a nutritional deficit. A panel of 25 simple sequence repeat (SSR) markers was utilised to assess the genetic diversity within four V. variegata populations, including individuals from Manombo and three other populations in southeastern Madagascar. The data revealed that the level of genetic diversity apparent within the Manombo population is comparable to the other groups studied. Therefore, it seems probable that genetic factors are not responsible for the recent lack of reproductive success on the part of the V. variegata at Manombo Special Reserve.