Skip to main content Accessibility help
×
Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-08T14:17:48.361Z Has data issue: false hasContentIssue false

1 - Adaptive Optical Microscopy Using Image-Based Wavefront Sensing

from Part I - Adaptive Optical Microscopy for Biological Imaging

Published online by Cambridge University Press:  10 June 2019

Joel Kubby
Affiliation:
University of California, Santa Cruz
Sylvain Gigan
Affiliation:
Sorbonne Université and Laboratoire Kastler-Brossel
Meng Cui
Affiliation:
Purdue University, Indiana
HTML view is not available for this content. However, as you have access to this content, a full PDF is available via the 'Save PDF' action button.

Summary

The general approach to wavefront sensorless adaptive optics (or “sensorless AO” for short) relies upon the optimisation of a measurement that is known to be related to the aberration content. For example, in an adaptive laser focussing system, one might maximise the intensity at the centre of the focussed beam. In image-based AO systems, which are a sub-category of sensorless AO systems, an appropriately chosen image property is optimised. In certain microscopes (e.g. confocal or multi-photon microscopes) the total image intensity (sum of all pixel values) is an appropriate optimisation metric, as it exhibits a maximum value when no aberration is present. If the aberration in the system is non-zero due to refractive index variations in the specimen, then the value of the metric will be lower than its optimal value. The goal of the sensorless AO routine would be to use the adaptive element to maximise the metric by minimising the total aberration in the system.
Type
Chapter
Information
Publisher: Cambridge University Press
Print publication year: 2019

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×