Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T05:02:16.090Z Has data issue: false hasContentIssue false

Chapter 19 - Gamete Preparation and Embryo Culture

Published online by Cambridge University Press:  03 June 2019

By
Karen Schnauffer
Edited by
Jane A. Stewart
Jane A. Stewart
Affiliation:
Newcastle Hospitals NHS Foundation Trust
Get access

Summary

The pioneers of in vitro fertilisation (IVF) were Robert Edwards, a scientist working at Cambridge University, and Patrick Steptoe, a gynaecologic surgeon from Oldham, who collaborated together to develop the techniques required to collect human eggs from the ovary and fertilise them in the laboratory. The birth of Louise Brown in 1978 is regarded as the single most important milestone in the world of assisted conception as it revealed that babies could be born as a result of eggs and sperm being mixed together in a laboratory to create embryos which were then transferred back into the patient’s uterus to create a pregnancy; a process known as IVF [1].

Type
Chapter
Information
Subfertility, Reproductive Endocrinology and Assisted Reproduction , pp. 146 - 152
Publisher: Cambridge University Press
Print publication year: 2019

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Steptoe, PC, Edwards, RG. Birth after the reimplantation of a human embryo. Lancet. 1978; 12(8085):366.CrossRefGoogle Scholar
Quinn, P, Kerin, JF, Warnes, GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril. 1985 Oct;44(4):493–498.CrossRefGoogle Scholar
Commission Directive 2006/17/EC of 8 February 2006 implementing Directive 2004/23/EC of the European Parliament and of the Council as regards certain technical requirements for the donation, procurement and testing of human tissues and cells.Google Scholar
Pickering, SJ, Braude, PR, Johnson, MH, Cant, A, Currie, J. Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril. 1990;54:102–108.Google Scholar
Roijen, JH, Slob, AK, Gianotten, WL, Dohle, GR, van der Zon, ATM, Vreeburg, JTM., Weber, RFA. Sexual arousal and the quality of semen produced by masturbation. Hum Reprod. 1996;11(1):147–151.Google Scholar
Kilgallon, SJ, Simmons, LW. Image content influences men’s semen quality. Biol Lett. 2005;22(3):253–255.Google Scholar
Pound, N, Javed, MH, Ruberto, C, Shaikh, MA, Del Valle, AP. Duration of sexual arousal predicts semen parameters for masturbatory ejaculates. Physiol Behav. 2002;76:685–689.Google Scholar
WHO. Laboratory manual for the examination and processing of human semen 5th edition, 2010.Google Scholar
Zinamen, M, Brown, C, Selevan, G, Clegg, E. Semen quality and human fertility: a prospective study with healthy couples. J Androl. 2000;21(1): 14–53.Google Scholar
Menkveld, R, Stander, FS, Kotze, TJ, Kruger, TF, van Zyl, JA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod. 1990 Jul;5(5):586–592.Google Scholar
HFEA. One Child at a Time, 2006. https://ifqlive.blob.core.windows.net/umbraco-website/1311/one-child-at-a-time-report.pdf.Google Scholar
Meseguer, M, Herrero, J, Tejera, A, Hilligsøe, K, Ramsing, NB, Remohı, J. The use of morphokinetics as a predictor of embryo implantation. Hum Reprod. 2011;26(10):2658–2671.Google Scholar
Rubio, I, Galán, A, Larreategui, Z, Ayerdi, F, Bellver, J, Herrero, J, Meseguer, M. Clinical validation of embryo culture and selection by morphokinetic analysis: a randomized, controlled trial of the EmbryoScope. Fertil Steril. 2014 Nov;102(5):1287–1294.Google Scholar
Barrie, A, Schnauffer, K, Kingsland, C, Troup, S. Treatment outcome and early pregnancy loss – a comparison of conventional and embryoscope® systems. Fertil Steril. 2013;100(3): Supplement, S248.CrossRefGoogle Scholar
Athayde Wirka, K, Chen, AA, Conaghan, J, Ivani, K, Gvakharia, M, Behr, B, Suraj, V, Tan, L, Shen, S. Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development. Fertil Steril. 2014 Jun;101(6):1637–1648.Google Scholar
Chavez, SL, Loewke, KE, Han, J, Moussavi, F, Colls, P, Munne, S, Behr, B, Reijo Pera, RA. Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage. Nat Commun. 2012;(3):1251.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×