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2 - Dicer in RNAi: Its roles in vivo and utility in vitro

Published online by Cambridge University Press:  31 July 2009

By
Jason W. Myers  and
James E. Ferrell Jr.
Edited by
Krishnarao Appasani
Foreword by
Andrew Fire  and
Marshall Nirenberg
Jason W. Myers
Affiliation:
Department of Molecular Pharmacology, Stanford University School of Medicine
James E. Ferrell Jr.
Affiliation:
Department of Molecular Pharmacology, Stanford University School of Medicine
Krishnarao Appasani
Affiliation:
GeneExpression Systems, Inc., Massachusetts
Andrew Fire
Affiliation:
Stanford University, California
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Summary

Introduction

In its infancy, RNA interference (RNAi) was simply an intriguing curiosity; now it has leapt to the forefront of science (Couzin, 2002). The growth spurt of RNAi-related studies has been partly pragmatic – RNAi clearly had the potential to evolve into an incredibly powerful technology for manipulating gene expression in diverse cell types, and to a great extent this potential has been realized. Moreover, as a physiological phenomenon, RNAi represents a fascinating and previously unrecognized level of cellular regulation. RNAi is essential for silencing of heterochromatin (Reinhart and Bartel, 2002; Volpe et al., 2002; Schramke and Allshire, 2003; Volpe et al., 2003) and gene expression via methylation (Grant, 1999; Jones et al., 1999), for antiviral defense, at least in plants (Baulcombe, 1999; Grant, 1999; Ratcliff et al., 1999), for controlling the expression of transposable elements and repetitive sequences (Ketting et al., 1999; Tabara et al., 1999; Ambros et al., 2003a&b; Sijen and Plasterk, 2003), and for proper embryonic development (Grishok et al., 2001; Hütvagner et al., 2001; Ketting et al., 2001; Knight and Bass, 2001; Bernstein et al., 2003; Houbaviy et al., 2003).

This chapter focuses on Dicer, an RNAse III family enzyme essential for sequence-specific gene suppression. We begin with an overview of the discovery of Dicer and its implication in RNAi, followed by a discussion of its hypothesized roles in vivo.

Type
Chapter
Information
RNA Interference Technology
From Basic Science to Drug Development
 , pp. 29 - 54
Publisher: Cambridge University Press
Print publication year: 2005

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