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Chapter 33 - Trophectoderm Biopsy for Pre-implantation Genetic Testing

from Section 6 - Embryo Assessment: Morphology and Beyond

Published online by Cambridge University Press:  07 August 2023

By
Maurizio Poli ,
Catello Scarica ,
Federico Favero ,
Laura Sosa Fernandez  and
Antonio Capalbo
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Trophectoderm (TE) biopsy is the gold standard for collecting embryo specimens for pre-implantation genetic testing (PGT). Because TE biopsies contain approximately 5–10 cells they provide a more robust template for DNA amplification, increasing diagnostic efficiency and representativeness of the whole embryo chromosomal constitution compared to a single cell biopsy. TE biopsy requires superior micromanipulation skills. Technical aspects include the combination of laser technology and micromanipulation to detach the biopsied specimen from the blastocyst. TE biopsy can be achieved employing different approaches according to the blastocyst’s characteristics and laboratory setting. Biopsy tubing entails a high degree of manual skills and coordination. In order to reach a highly efficient PGT program, blastocyst culture, biopsy and tubing technique, as well as robust cryopreservation processes are required.

Keywords

PGTbiopsytrophectodermmicromanipulation
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 240 - 250
Publisher: Cambridge University Press
Print publication year: 2023

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References

Scott, R. T., Upham, K. M., Forman, E. J., Zhao, T. and Treff, N. R. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril 2013; 100(3):624–30.Google Scholar
Tiegs, A. W., Tao, X., Zhan, Y., et al. A multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing-based preimplantation genetic testing for aneuploidy assay and impact of biopsy. Fertil Steril 2021; 115(3):627–37.CrossRefGoogle ScholarPubMed
Cimadomo, D., Ubaldi, F. M., Capalbo, A., et al. Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles. Reprod Biomed Online 2016; 33(3):360–9.Google Scholar
Capalbo, A., Ubaldi, F. M., Cimadomo, D., et al. Consistent and reproducible outcomes of blastocyst biopsy and aneuploidy screening across different biopsy practitioners: a multicentre study involving 2586 embryo biopsies. Hum Reprod 2016; 31(1):199–208.Google Scholar
McArthur, S. J., Leigh, D., Marshall, J. T., de Boer, K. A. and Jansen, R. P. S. Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts. Fertil Steril 2005; 84(6):1628–36.Google Scholar
Capalbo, A., Rienzi, L., Cimadomo, D., et al. Correlation between standard blastocyst morphology, euploidy and implantation: an observational study in two centers involving 956 screened blastocysts. Hum Reprod 2014; 29(6):1173–81.CrossRefGoogle ScholarPubMed
ESHRE PGT Consortium Steering Committee C, Carvalho, F., Coonen, E., et al. ESHRE PGT Consortium good practice recommendations for the organisation of PGT. Hum Reprod Open 2020; 2020(3):hoaa021.CrossRefGoogle ScholarPubMed
Cimadomo, D., Rienzi, L., Romanelli, V., et al. Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience. Hum Reprod 2018; 33(10):1839–46.Google Scholar

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