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Chapter 23 - Technical Aspects of ICSI for Ejaculated Spermatozoa

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Derek Keating ,
Mounia Haddad ,
Stephanie Cheung ,
Phillip Xie ,
Alessandra Parrella  and
Gianpiero D. Palermo
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Intracytoplasmic sperm injection (ICSI) was first described in 1992 as the ultimate tool for treating couples plagued by male factor infertility. Since then, it has become the most prevalent assisted reproductive technology (ART) treatment in the world, due to its incredible versatility and expanding cohort of indications for use. ICSI requires both a dedicated laboratory setup and knowledgeable and skilled embryologists in order to competently treat infertile couples. In this chapter, we will briefly describe the procedure, indications, required equipment and proper protocol in order to achieve reliable ICSI results and maintain consistent clinical outcome.

Keywords

ICSIsperm injectionfertilizationsperm
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 170 - 177
Publisher: Cambridge University Press
Print publication year: 2023

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References

Cohen, J., Edwards, R. G., Fehilly, C. B., et al. Treatment of male infertility by in vitro fertilization: factors affecting fertilization and pregnancy. Acta Eur Fertil 1984; 15:455–65.Google ScholarPubMed
Nyboe Andersen, A., Carlsen, E. and Loft, A. Trends in the use of intracytoplasmatic sperm injection marked variability between countries. Hum Reprod Update 2008; 14:593–604.CrossRefGoogle ScholarPubMed
Adamson, G. D., de Mouzon, J., Chambers, G. M., et al. International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology, 2011. Fertil Steril 2018; 110:1067–80.CrossRefGoogle ScholarPubMed
Fishel, S., Aslam, I., Lisi, F., et al. Should ICSI be the treatment of choice for all cases of in-vitro conception? Hum Reprod 2000; 15:1278–83.CrossRefGoogle ScholarPubMed
Aboulghar, M. A., Mansour, R. T., Serour, G. I., Sattar, M. A. and Amin, Y. M. Intracytoplasmic sperm injection and conventional in vitro fertilization for sibling oocytes in cases of unexplained infertility and borderline semen. J Assist Reprod Genet 1996; 13:38–42.CrossRefGoogle ScholarPubMed
Palermo, G. D., Neri, Q. V., Monahan, D., Kocent, J. and Rosenwaks, Z. Development and current applications of assisted fertilization. Fertil Steril 2012; 97:248–59.CrossRefGoogle ScholarPubMed
Moomjy, M., Sills, E. S., Rosenwaks, Z. and Palermo, G. D. Implications of complete fertilization failure after intracytoplasmic sperm injection for subsequent fertilization and reproductive outcome. Hum Reprod 1998; 13:2212–6.CrossRefGoogle ScholarPubMed
Van Steirteghem, A. C., Nagy, Z., Joris, H., et al. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993; 8:1061–6.CrossRefGoogle ScholarPubMed
Nagy, Z. P., Liu, J., Joris, H., et al. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 1995; 10:1123–9.CrossRefGoogle ScholarPubMed
Tournaye, H., Liu, J., Nagy, Z., et al. The use of testicular sperm for intracytoplasmic sperm injection in patients with necrozoospermia. Fertil Steril 1996; 66:331–4.Google ScholarPubMed
Nagy, Z. P., Verheyen, G., Liu, J., et al. Results of 55 intracytoplasmic sperm injection cycles in the treatment of male-immunological infertility. Hum Reprod 1995; 10:1775–80.CrossRefGoogle ScholarPubMed
Liu, J., Nagy, Z., Joris, H., et al. Successful fertilization and establishment of pregnancies after intracytoplasmic sperm injection in patients with globozoospermia. Hum Reprod 1995; 10:626–9.CrossRefGoogle ScholarPubMed
Lundin, K., Sjogren, A., Nilsson, L. and Hamberger, L. Fertilization and pregnancy after intracytoplasmic microinjection of acrosomeless spermatozoa. Fertil Steril 1994; 62:1266–7.CrossRefGoogle ScholarPubMed
Bourne, H., Richings, N., Harari, O., et al. The use of intracytoplasmic sperm injection for the treatment of severe and extreme male infertility. Reprod Fertil & Dev 1995; 7:237–45.CrossRefGoogle ScholarPubMed
Palermo, G. D., O’Neill, C. L., Chow, S., et al. Intracytoplasmic sperm injection: state of the art in humans. Reproduction 2017; 154(6):F93–110.CrossRefGoogle ScholarPubMed
O’Neill, C. L., Parrella, A., Keating, D., et al. A treatment algorithm for couples with unexplained infertility based on sperm chromatin assessment. J Assist Reprod Genet 2018; 35:1911–17.CrossRefGoogle ScholarPubMed
Casanovas, A., Ribas-Maynou, J., Lara-Cerrillo, S., et al. Double-stranded sperm DNA damage is a cause of delay in embryo development and can impair implantation rates. Fertil Steril 2019; 111:699–707.CrossRefGoogle ScholarPubMed
Parella, A., Keating, D., Cheung, S., et al. A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure. J Assist Reprod Genet 2019; 36(10):2057–66.Google Scholar
Maggiulli, R., Neri, Q. V., Monahan, D., et al. What to do when ICSI fails. Syst Biol Reprod Med 2010; 56:376–87.CrossRefGoogle Scholar
Pereira, N., Neri, Q. V., Lekovich, J. P., Palermo, G. D. and Rosenwaks, Z. The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity. Reprod Biomed Online. 2016; 32(4):401–6.CrossRefGoogle ScholarPubMed
Palermo, G. D., Cohen, J., Alikani, M., Adler, A. and Rosenwaks, Z. Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil Steril 1995; 63:1231–40.CrossRefGoogle ScholarPubMed
World Health Organization (WHO). Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. (Cambridge: Cambridge University Press, 2010).Google Scholar
Pereira, N., Reichman, D. E., Goldschlag, D. E., Lekovich, J. P. and Rosenwaks, Z. Impact of elevated peak serum estradiol levels during controlled ovarian hyperstimulation on the birth weight of term singletons from fresh IVF-ET cycles. J Assist Reprod Genet 2015; 32:527–32.CrossRefGoogle ScholarPubMed
Huang, J. Y. and Rosenwaks, Z. In vitro fertilisation treatment and factors affecting success. Best Pract Res Clin Obstet Gynaecol 2012; 26:777–88.CrossRefGoogle ScholarPubMed
Palermo, G. D., Schlegel, P. N., Colombero, L. T., et al. Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum Reprod 1996; 11:1023–9.CrossRefGoogle ScholarPubMed

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