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Chapter 12 - Quality Control in the IVF Laboratory

Continuous Improvement

from Section 2 - Pre-procedure Protocols

Published online by Cambridge University Press:  07 August 2023

By
Georgios Liperis  and
Cecilia Sjöblom
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

The performance of the embryology laboratory is of imperative importance for the successful outcome of an assisted reproductive technology (ART) cycle. The development and viability of gametes and embryos can be compromised by small fluctuations in their environment, so it is crucial to establish optimal culture conditions at which gametes and embryos are attained and maintained. Having a robust control of culture conditions means that it is not necessary to search for a needle in a haystack when trouble-shooting. In order to ensure and maintain these very specific conditions, quality control (QC) routines need to be established with quality specifications for each quality parameter. These parameters are assessed on a daily, weekly, monthly or annual basis to determine whether they meet certain specifications, followed by any necessary fine-tuning to bring the levels back within the acceptable limits of uncertainty of measurement.

Keywords

quality controlquality assurancequality improvementquality managementIVF
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 86 - 95
Publisher: Cambridge University Press
Print publication year: 2023

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References

Wikland, M. and Sjoblom, C. The application of quality systems in ART programs. Mol Cell Endocrinol 2000; 166:3–7.CrossRefGoogle ScholarPubMed
Brinster, R. L. In vitro cultivation of mammalian ova. Adv Biosci 1969; 4:199–233.Google Scholar
Almeida, P. A. and Bolton, V. N. The effect of temperature fluctuations on the cytoskeletal organization and chromosomal constitution of the human oocyte. Zygote 1995; 3(4):357–65.CrossRefGoogle ScholarPubMed
Higdon, H. L., 3rd, Blackhurst, D. W. and Boone, W. R. Incubator managements in an assisted reproductive technology laboratory. Fertil Steril 2008; 89(3):703–10.CrossRefGoogle Scholar
Swain, J. E. Optimizing the culture environment in the IVF laboratory: impact of pH and buffer capacity on gamete and embryo quality. Reprod Biomed Online 2010; 21:6–16.CrossRefGoogle ScholarPubMed
Swain, J. E. and Pool, T. B. New pH-buffering system for media utilized during gamete and embryo manipulations for assisted reproduction. Reprod. Biomed Online 2009; 18:799–810.CrossRefGoogle ScholarPubMed
Koustas, G. and Sjoblom, C. Epigenetic consequences of pH stress in mouse embryos. Hum Reprod 2011; 26:i78.Google Scholar
Fitzharris, G. and Baltz, J. Regulation of intracellular pH during oocyte growth and maturation in mammals. Reproduction 2009; 138:619–27.CrossRefGoogle ScholarPubMed
Squirrell, J. M., Lane, M. and Bavister, B. D. Altering intracellular pH disrupts development and cellular organization in preimplantation hamster embryos. Biol Reprod 2001; 64:1845–54.CrossRefGoogle ScholarPubMed
Swain, J. E. Is there an optimal pH for culture media used in clinical IVF? Hum Reprod Update 2012; 18(3):333–9.CrossRefGoogle ScholarPubMed
Quinn, P. and Cooke, S. Equivalency of culture media for human in vitro fertilization formulated to have the same pH under an atmosphere containing 5% or 6% carbon dioxide. Fertil Steril 2004; 81:1502–6.Google Scholar
Graves, C. N. and Biggers, J. D. Carbon dioxide fixation by mouse embryos prior to implantation. Science 1970; 167:1506–8.CrossRefGoogle ScholarPubMed
Peng, H., Shi, W., Zhang, W., et al. Better quality and more usable embryos obtained on day 3 cultured in 5% than 20% oxygen: a controlled and randomized study using the sibling oocytes. Reprod Sci 2015; pii:1933719115602761.CrossRefGoogle Scholar
Morbeck, D. E. Air quality in the assisted reproduction laboratory: a mini-review. J Assist Reprod Genet 2015; 32(7):1019–24.CrossRefGoogle ScholarPubMed
Boone, W. R., Johnson, J. E., Locke, A. J., Crane, M. M., 4th and Price, T. M. Control of air quality in an assisted reproductive technology laboratory. Fertil Steril 1999; 71(1):150–4.Google Scholar
Directive 2003/23/EC of the European Parliament and of the Council of 31 March 2004 on setting standards of quality and safety for the donation, procurement, testing, processing, preservation, storage and distribution of human tissues and cells. Official Journal of the European Union, L 102, 7.4.2004, pp. 48–58, available at http://europa.eu.int/eur-lex/en/oj/.Google Scholar

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