Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-05T22:34:37.291Z Has data issue: false hasContentIssue false

Chapter 10 - Mouse Embryo Assay for Quality Control in the IVF Laboratory

from Section 2 - Pre-procedure Protocols

Published online by Cambridge University Press:  07 August 2023

By
Dean E. Morbeck
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
Get access

Summary

Ensuring proper quality control (QC) in the laboratory is critical to the success of any in vitro fertilization (IVF) programme, as the environment of the laboratory can alter the quality of the embryos produced. The ultimate role of the embryology laboratory is to maintain the inherent viability of the gametes and embryos in an environment outside the female reproductive tract. There is a need for objective, sensitive and reproducible methods and assays for testing materials for embryo toxicity as well as growth promoting and inhibiting factors. The manufacturers test commercially available IVF media and plastic ware and provide the results of their testing with the shipment of supplies. It may be advisable to test media and supplies upon arrival to confirm that nothing occurred during shipping. The suitability of various reagents and materials for use in human IVF can be tested using a range of bioassays. While the most used bioassays for QC in IVF laboratories are the human sperm survival assay and the mouse embryo bioassay (MEA), the one-cell MEA has been consistently shown to be the most sensitive.

Keywords

quality controlmouse embryotoxicity
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 75 - 79
Publisher: Cambridge University Press
Print publication year: 2023

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bavister, B. D. Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update 1995; 1(2):91–148.CrossRefGoogle ScholarPubMed
Leese, H. J. Metabolic control during preimplantation mammalian development. Hum Reprod Update 1995; 1:63–72.CrossRefGoogle ScholarPubMed
Punt–van der Zalm, J. P. E. M., Hendriks, J. C. M., Westphal, J. R., et al. Toxicity testing of human assisted reproduction devices using the mouse embryo assay. Repro Biomed Online 2009; 18(4):529–35.Google Scholar
Lane, M. and Gardner, D. K. Differential regulation of mouse embryo development and viability by amino acids. J Reprod Fertil 1997; 109:153–64.Google ScholarPubMed
Davidson, A., Vermesh, M., Lobo, R. and Paulson, R. Mouse embryo culture as quality control for human in vitro fertilization: the one-cell versus the two-cell model. Fertil Steril 1988; 49:516–21.CrossRefGoogle Scholar
Hughes, P. M., Morbeck, D. E., Hudson, S., et al. Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays. J Assist Reprod Genet 2010; 27:87–92.CrossRefGoogle ScholarPubMed
Mestres, E., Garcia-Jiménez, M., Faes, L., et al. Parameters of the mouse embryo assay that affect detection of peroxides in mineral oil. Reprod Biomed Online 2019; 39:547–55.Google Scholar
Ainsworth, A. J., Fredrickson, J. R. and Morbeck, D.E. Improved detection of mineral oil toxicity using an extended mouse embryo assay. J Assist Reprod Genet 2017; 34:391–7.CrossRefGoogle ScholarPubMed
Scott, L. F., Sundaram, S. G. and Smith, S. The relevance and use of mouse embryo bioassays for quality control in an assisted reproductive technology program. Fertil Steril 1993; 60:559–68.CrossRefGoogle Scholar
Morbeck, D.E., Khan, Z., Barnidge, D. R. and Walker, D. L. Washing mineral oil reduces contaminants and embryotoxicity. Fertil Steril 2010; 94:2747–52.Google Scholar
Suzuki, O., Asano, T., Yamamoto, Y., Takano, K. and Koura, M. Development in vitro of preimplantation embryos from 55 mouse strains. Reprod Fertil Dev 1996; 8:975–80.Google Scholar
Khan, Z., Morbeck, D. E., Walker, D. L. et al. Mouse embryos and in vitro stress: does mouse strain matter? Fertil Steril 2010; 94:S58.CrossRefGoogle Scholar
Gardner, D. K., Reed, L., Linck, D., Sheehan, C. and Lane, M. Quality control in human in vitro fertilization. Semin Reprod Med 2005; 23(4):319–24.CrossRefGoogle ScholarPubMed
Wolff, H. S., Fredrickson, J. R., Walker, D. L. and Morbeck, D. E. Advances in quality control: mouse embryo morphokinetics are sensitive markers of in vitro stress. Hum Reprod 2013; 28(7):1776–82.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×