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Chapter 21 - General Insemination for IVF

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Caroline McCaffrey ,
Melicia Clarke-Williams  and
David H. McCulloh
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Appropriate procedures must be documented that describe all the steps performed during an insemination of oocytes for in vitro fertilization (IVF). These procedures must be designed bothe to optimize the fertilization of oocytes and to prevent circumstances that increase the risk of introduction, transmission or spread of communicable diseases through the collection, preparation, culture and/or transfer of patients’ gametes and embryos.

Keywords

IVFinseminationspermoocytefertilization
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 159 - 165
Publisher: Cambridge University Press
Print publication year: 2023

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References

Tanghe, S., Van Soom, A., Nauwynck, H., Coryn, M. and De Kruif, A. Minireview: functions of the cumulus oophorus during oocyte maturation, ovulation and fertilization. Mol Reprod & Dev 2002; 61:414–24.Google Scholar
McCulloh, D. H., Goorbarry, J., Shah, S. and Ahmad, K. Oocyte lysis following intracytoplasmic sperm injection: association with measures of oocyte quality and technician performance. J Reprod Stem Cell Biotech 2011; 2(1):46–54.Google Scholar
World Health Organization (WHO). Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction, 4th ed. (Cambridge: Cambridge University Press, 2004).Google Scholar
Keegan, B. R., Barton, S., Sanchez, S., et al. Isolated teratozoospermia does not affect in vitro fertilization outcome and is not an indication for intracytoplasmic sperm injection. Fertil Steril 2007; 88 (6):1583–8.CrossRefGoogle Scholar

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