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Chapter 28 - Fertilization Assessment in IVF and ICSI

from Section 5 - Fertilization Assessment

Published online by Cambridge University Press:  07 August 2023

By
Dara S. Berger  and
Heather S. Hoff
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Selection of the best embryo to transfer, that is, the one that will have the highest potential to result in a live birth, is one of the most important decisions in an in vitro fertilization (IVF) laboratory. A zygote forms with the amalgamation of the female and male gametes, and much information about the viability of an embryo can be gained within 16 to 20 hours after insemination. Interaction of the oocyte and sperm should first be examined with an accurate assessment of fertilization.

Keywords

IVFICSIfertilization2PNzygote
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 207 - 212
Publisher: Cambridge University Press
Print publication year: 2023

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References

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Alpha Scientists in Reproductive Medicine and European Society of Human Reproduction and Embryology. Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Reprod Biomed Online 2011; 22(6):632–46.Google Scholar
Plachot, M., Mandelbaum, J., Junca, A. M., et al. Co-culture with granulosa cells does not increase the fertilization rate in couples with previous fertilization failures. Hum Reprod 1993; 8(9):1455–8.CrossRefGoogle Scholar
Hondo, S., Arichi, A., Muramatsu, H., et al. Clinical outcomes of transfer of frozen and thawed single blasocysts derived from nonpronuclear and monpronuclear zygotes. Reprod Med Biol 2019; 18:278–83.CrossRefGoogle Scholar
Valeria Paz, M., Chiera, M., Hovanyecz, P., et al. Blastocysts derived from 0PN oocytes: Genetic and clinical results. JBRA Assist Reprod 2020; 24(2):143–6.Google Scholar
Grau, N. Escrich, L., Martín, J., et al. Self-correction in tripronucleated human embryos. Fertil Steril 2011; 96(4):951–6.CrossRefGoogle ScholarPubMed

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