The design and development of perfusion cultures require extensive experiental campaigns in order to identify the most convenient conditions in terms of a very large number of operating parameters. There is a need for high-throughput technologies that allow for the simultaneous operation and monitoring of several cultures at different process conditions. This requires suitable scale-down models, which allow the reliable prediction of the behaviour at larger scale. Within this chapter, we describe systems allowing the operation at the µL and mL scale and enabling early process development, such as clone and media screening, as well as the design of suitable process operating conditions at the larger scale. Managing such a high number of experiments is not possible without a proper level of automation, including suitable sensors for process monitoring.