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Chapter 24 - The Current Use of Sperm Function Assays

from Section 4 - Laboratory Evaluation and Treatment of Male Infertility

Published online by Cambridge University Press:  06 December 2023

By
Sara Marchiani  and
Elisabetta Baldi
Edited by
Douglas T. Carrell ,
Alexander W. Pastuszak  and
James M. Hotaling
Douglas T. Carrell
Affiliation:
Utah Center for Reproductive Medicine
Alexander W. Pastuszak
Affiliation:
University of Utah
James M. Hotaling
Affiliation:
Utah Center for Reproductive Medicine
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Summary

Although semen analysis is the main routine test used in the diagnosis of male infertility, it is considered to be poorly predictive of male fertility status. Only recently, research in sperm biology focused on the development of assays to evaluate sperm functions necessary to reach and fertilize the oocyte and to allow a correct embryo development. However, the clinical utility of the currently proposed tests/assays remains a matter of debate, especially after introduction of ICSI. Ideally, since fertilization proceeds in a cascade-like manner, the goal would be to develop a single test able to evaluate all the aspects involved in this process and introduce it in ART laboratories. Such test should be simple, cheap, and not requiring expensive technology. Unfortunately, this aim is still far from being achieved. In this chapter, we discuss the most promising tests assessing sperm functions, describing their validity, limits, and potential use in clinical practice.

Keywords

men’s healthreproductionsemen analysissperm capacitationARTICSICASAhyperactivated spermHemizona assayacrosome functionsperm-oocyte fusionsperm chromatinsperm DNA
Type
Chapter
Information
Men's Reproductive and Sexual Health Throughout the Lifespan
An Integrated Approach to Fertility, Sexual Function, and Vitality
 , pp. 191 - 196
Publisher: Cambridge University Press
Print publication year: 2023

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References

Baskaran, S, Finelli, R, Agarwal, A, Henkel, R. Diagnostic value of routine semen analysis in clinical andrology. Andrologia. 2020;12:e13614.Google Scholar
Eisenberg, ML, Li, S, Behr, B, Pera, RR, Cullen, MR. Relationship between semen production and medical comorbidity. Fertil Steril. 2015;103:66–71.Google Scholar
Wen, J, Jiang, J, Ding, C, et al. Birth defects in children conceived by in vitro fertilization and intracytoplasmic sperm injection: a meta-analysis. Fertil Steril. 2012;97(6):1331–1337.CrossRefGoogle ScholarPubMed
Sakkas, D, Ramalingam, M, Garrido, N, Barratt, CL. Sperm selection in natural conception: what can we learn from Mother Nature to improve assisted reproduction outcomes? Hum Reprod Update. 2015;21:711–726.Google Scholar
Baldi, E, Tamburrino, L, Muratori, M, Degl’Innocenti, S, Marchiani, S. Adverse effects of in vitro manipulation of spermatozoa. Anim Reprod Sci. 2020;14:106314.CrossRefGoogle Scholar
Davies, MJ, Moore, VM, Willson, KJ, et al. Reproductive technologies and the risk of birth defects. N Engl J Med. 2012;366:1803–1813.Google Scholar
Bonduelle, M, Wennerholm, UB, Loft, A, et al. A multi-centre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Hum Reprod. 2005;20:413–419.CrossRefGoogle ScholarPubMed
Puga Molina, LC, Luque, GM, Balestrini, PA, Marín-Briggiler, CI, Romarowski, A, Buffone, MG. Molecular basis of human sperm capacitation. Front Cell Dev Biol. 2018;6:72.Google Scholar
Baldi, E, Casano, R, Falsetti, C, Krausz, C, Maggi, M, Forti, G. Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa. J Androl. 1991;12:323–330.Google Scholar
Krausz, C, Bonaccorsi, L, Maggio, P, et al. Two functional assays of sperm responsiveness to progesterone and their predictive values in in-vitro fertilization. Hum Reprod. 1996;11:1661–1667.Google Scholar
Baro Graf, C, Ritagliati, C, Torres-Monserrat, V, et al. Membrane potential assessment by fluorimetry as a predictor tool of human sperm fertilizing capacity. Front Cell Dev Biol. 2020;7:383.CrossRefGoogle ScholarPubMed
Selvaraj, V, Buttke, DE, Asano, A, et al. GM1 dynamics as a marker for membrane changes associated with the process of capacitation in murine and bovine spermatozoa. J Androl. 2007;28:588–599.CrossRefGoogle ScholarPubMed
Moody, MA, Cardona, C, Simpson, AJ, Smith, TT, Travis, AJ, Ostermeier, GC. Validation of a laboratory-developed test of human sperm capacitation. Mol Reprod Dev. 2017;84:408–422.Google Scholar
Ostermeier, GC, Cardona, C, Moody, MA, et al. Timing of sperm capacitation varies reproducibly among men. Mol Reprod Dev. 2018;85:387–396.Google Scholar
Cardona, C, Neri, QV, Simpson, AJ, et al. Localization patterns of the ganglioside GM1 in human sperm are indicative of male fertility and independent of traditional semen measures. Mol Reprod Dev. 2017;84:423–435.Google Scholar
Sharara, F, Seaman, E, Morris, R, et al. Multicentric, prospective observational data show sperm capacitation predicts male fertility, and cohort comparison reveals a high prevalence of impaired capacitation in men questioning their fertility. Reprod Biomed Online. 2020;41:69–79.Google Scholar
Mortimer, ST, van der Horst, G, Mortimer, D. The future of computer-aided sperm analysis. Asian J Androl. 2015;17:545–553.CrossRefGoogle ScholarPubMed
Katz, DF, Overstreet, JW. Sperm motility assessment by videomicrography. Fertil Steril. 1981;35:188–193.CrossRefGoogle ScholarPubMed
Sukcharoen, N, Keith, J, Irvine, DS, Aitken, RJ. Definition of the optimal criteria for identifying hyperactivated human spermatozoa at 25 Hz using in-vitro fertilization as a functional end-point. Hum Reprod. 1995;10:2928–2937.Google Scholar
Mortimer, D, Mortimer, ST. Computer-aided sperm analysis (CASA) of sperm motility and hyperactivation. Methods Mol Biol. 2013;927:77–87.Google Scholar
Senn, A, Germond, M, De Grandi, P. Immunofluorescence study of actin, acrosin, dynein, tubulin and hyaluronidase and their impact on in-vitro fertilization. Hum Reprod. 1992;7:841–849.Google Scholar
Sharma, R, Hogg, J, Bromham, DR. Is spermatozoan acrosin a predictor of fertilization and embryo quality in the human? Fertil Steril. 1993;60:881–887.Google Scholar
Menkveld, R, Rhemrev, JP, Franken, DR, Vermeiden, JP, Kruger, TF. Acrosomalmorphology as a novel criterion for male fertility diagnosis: relation with acrosin activity, morphology (strict criteria), and fertilization in vitro. Fertil Steril. 1996;65:637–644.Google Scholar
Yang, YS, Chen, SU, Ho, HN, et al. Acrosin activity of human sperm did not correlate with IVF. Arch Androl. 1994;32:13–19.Google Scholar
Liu, DY, Baker, HWG. Relationships between human sperm acrosin, acrosomes, morphology and fertilization in vitro. Hum Reprod. 1990;5:298–303.Google Scholar
Parinaud, J, Vieitez, G, Moutaffian, H, Richoilley, G, Labal, B. Variations in spontaneous and induced acrosome reaction: correlations with semen parameters and in-vitro fertilization results. Hum Reprod. 1995;10:2085–2089.CrossRefGoogle ScholarPubMed
Xu, F, Guo, G, Zhu, W, Fan, L. Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis. Reprod Biol Endocrinol. 2018;16:81.Google Scholar
Xu, F, Zhu, H, Zhu, W, Fan, L. Human sperm acrosomal status, acrosomal responsiveness, and acrosin are predictive of the outcomes of in vitro fertilization: a prospective cohort study. Reprod Biol. 2018;18:344–354.Google Scholar
Chen, X, Zheng, Y, Zheng, J, Lin, J, Zhang, L, Jin, J. The progesterone-induced sperm acrosome reaction is a good option for the prediction of fertilization in vitro compared with other sperm parameters. Andrologia. 2019;51:e13278.CrossRefGoogle ScholarPubMed
Jin, M, Fujiwara, E, Kakiuchi, Y, et al. Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. Proc Natl Acad Sci U S A. 2011;108:4892–4896.Google Scholar
Gahlay, GK, Rajput, N. The enigmatic sperm proteins in mammalian fertilization: an overview. Biol Reprod. 2020;103(6):1171–1185.Google Scholar
Burkman, LJ, Coddington, CC, Franken, DR, Krugen, TF, Rosenwaks, Z, Hogen, GD. The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril. 1988;49:688–697.Google Scholar
Liu, DY, Baker, HW. High frequency of defective sperm–zona pellucida interaction in oligozoospermic infertile men. Hum Reprod. 2004;19:228–233.CrossRefGoogle ScholarPubMed
Yao, YQ, Yeung, WS, Ho, PC. The factors affecting sperm binding to the zona pellucida in the hemizona binding assay. Hum Reprod. 1996;11:1516–1519.Google Scholar
Vogiatzi, P, Chrelias, C, Cahill, DJ, et al. Hemizona assay and sperm penetration assay in the prediction of IVF outcome: a systematic review. Biomed Res Int. 2013;2013:945825.Google Scholar
Oehninger, S, Franken, DR, Sayed, EM, Barroso, G, Kohm, P. Sperm function assays and their predictive value for fertilization outcome in IVF therapy: a meta analysis. Hum Reprod Update. 2000;6:1160–1168.CrossRefGoogle ScholarPubMed
Aydin, H, Sultana, A, Li, S, Thavalingam, A, Lee, JE. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature. 2016;534:562–565.Google Scholar
Granados-Gonzalez, V, Aknin-Seifer, I, Touraine, RL, Chouteau, J, Wolf, JP, Levy, R. Preliminary study on the role of the human IZUMO gene in oocyte-spermatozoa fusion failure. Fertil Steril. 2008;90:1246–1248.Google Scholar
Yanagimachi, R, Yanagimachi, H, Rogers, BJ. The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod. 1976;15:471–476.CrossRefGoogle ScholarPubMed
Ford, WC, Williams, KM, Harrison, S, et al. Value of the hamster oocyte test and computerised measurements of sperm motility in predicting if four or more viable embryos will be obtained in an IVF cycle. Int J Androl. 2001;24:109–119.Google Scholar
Ho, LM, Lim, AS, Lim, TH, Hum, SC, Yu, SL, Kruger, TF. Correlation between semen parameters and the Hamster Egg Penetration Test (HEPT) among fertile and subfertile men in Singapore. J Androl. 2007;28:158–163.Google Scholar
Simon, L, Murphy, K, Shamsi, MB, et al. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod. 2014;29:2402e12.Google Scholar
Zini, A, Boman, JM, Belzile, E, et al. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis. Hum Reprod. 2008;23:2663e8.Google Scholar
Dutta, S, Henkel, R, Agarwal, A. Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation. Andrologia. 2020; 6:e13718.Google Scholar
Marchiani, S, Tamburrino, L, Muratori, M, Baldi, E. Spermatozoal chromatin structure: role in sperm functions and fertilization. In: Arafa, M, Elbardisi, H, Majzoub, A, Agarwal, A. eds. Genetics of Male Infertility: A Case-Based Guide for Clinicians. Springer; 2020:39–57.CrossRefGoogle Scholar
Marchiani, S, Tamburrino, L, Benini, F, et al. Chromatin protamination and CATSPER expression in spermatozoa predict clinical outcomes after assisted reproduction programs. Sci Rep. 2017;7:15122.Google Scholar
Simon, L, Zini, A, Dyachenko, A, Ciampi, A, Carrell, DT. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl. 2017;19:80–90.Google Scholar
Cissen, M, Wely, MV, Scholten, I, et al. Measuring sperm DNA fragmentation and clinical outcomes of medically assisted reproduction: a systematic review and meta-analysis. PLoS ONE. 2016;11:e0165125.CrossRefGoogle ScholarPubMed
World Health Organization. Laboratory Manual for the Examination and Processing of Human Semen. 5th ed. WHO Press; 2010.Google Scholar

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