Zona Binding: Hemizona Assay

doi:10.1017/9781108878715.015

Chapter 12 - Zona Binding: Hemizona Assay

Published online by Cambridge University Press: 05 April 2021

Shubhadeep Roychoudhury and

Edited by

Ralf Henkel and

Ashok Agarwal: Affiliation:
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel: Affiliation:
University of the Western Cape, South Africa
Ahmad Majzoub: Affiliation:
Hamad Medical Corporation, Doha

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Summary

The incidence of infertility is up to 20–25 percent in men with poor semen quality with a contribution of the male factor in 30–50 percent of couples undergoing assisted reproduction, including in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). In 17 studies sampling 6410 women, the proportion of couples seeking such medical care was, on average, 56.1 percent (range 42–76.3 percent) in more developed countries and 51.2 percent (range 27–74.1 percent) in less developed countries [1]. Investigation of male infertility or sub-fertility basically comprises of semen analysis [2]. However, a standard semen analysis cannot always assess the multifunctional events and biological properties that spermatozoa express following capacitation. In many cases, it is only when couples fail to achieve conception, the male factor is suspected and advanced laboratory tests are recommended to establish this reliably.

Type: Chapter
Information: Manual of Sperm Function Testing in Human Assisted Reproduction , pp. 100 - 105

DOI: https://doi.org/10.1017/9781108878715.015 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2021

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References

Boivin, J, Bunting, L, Collins, JA, Nygren, KG. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Hum Reprod 2007; 22: 1506–12.CrossRef Google Scholar PubMed

World Health Organization. (2010) WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva: The WHO Press.Google Scholar

Franken, DR, Kruger, TF, Oehninger, S, Coddington, CC, Lombard, C, Smith, K. The ability of the hemizona assay to predict human fertilization in different and consecutive in-vitro fertilization cycles. Hum Reprod 1993; 8: 1240–4.Google Scholar

Garrett, C, Liu, DY, Gordon Baker, HWG. Selectivity of the human sperm-zona pellucida binding process to sperm head morphometry. Fertil Steril 1997; 67: 362–71.Google Scholar

Oehninger, S, Toner, JP, Muasher, SJ, Coddington, C, Acosta, AA, Hodgen, GD. Prediction of fertilization in vitro with human gametes: is there a litmus test? Am J Obstet Gynecol 1992; 167: 1760–7.Google Scholar

Paul, M, Sumpter, JP, Lindsay, KS. The paradoxical effects of pentoxifylline on the binding of spermatozoa to the human zona pellucida. Hum Reprod 1996; 11: 814–19.CrossRef Google Scholar

Liu, DY, Baker, HW. Evaluation and assessment of semen for IVF/ICSI. Asian J Androl 2002; 4: 281–5.Google Scholar PubMed

Liu, DY, Lopata, A, Johnston, WI, Baker, HW. Human sperm zona pellucida binding, sperm characteristics and in-vitro fertilization. Hum Reprod 1989; 4: 696–701.Google Scholar

Liu, DY, Baker, HW. Defective sperm-zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization. Hum Reprod 2000; 15: 702–8.Google Scholar

Liu, DY, Baker, HW. Frequency of defective sperm-zona pellucida interaction in severely teratozoospermic infertile men. Hum Reprod 2003; 18: 802–7.Google Scholar

Oehninger, S, Coddington, CC, Scott, R, Franken, DR, Burkman, J, Acosta, AA, Hodgen, GD. Hemizon assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome. Fertil Steril 1989; 1: 665–70.Google Scholar

Dunbar, BS, Wolgemuth, DJ. Structure and function of the mammalian zona pellucida, a unique extracellular matrix. Mod Cell Biol 1984; 3: 77–111.Google Scholar

Nikas, G, Paraschos, T, Psychoyos, A, Handyside, AH. The zona reaction in human oocytes as seen with scanning electron microscopy. Hum Reprod 1994; 9: 2135–8.Google Scholar

Lefievre, L, Conner, SJ, Salpekar, A, Olufowobi, O, Ashton, P, Pavlovic, B. Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 2004; 19: 1580–6.CrossRef Google Scholar PubMed

Huszar, G, Vique, L, Oehninger, SC. Creatine kinase immunocytochemistry of human sperm-hemizona complexes: selective binding of sperm with mature creatine kinase staining pattern. Fertil Steril 1994; 61: 129–35.Google Scholar

Kruger, TF, Oehninger, S, Franken, DR, Hodgen, GD. Hemizona assay: use of fresh versus salt-stored human oocytes to evaluate sperm binding potential to the zona pellucida. J In Vitro Fert Embry Trans 1991; 8: 154–6.CrossRef Google Scholar

Huang, TT, Ohzu, E, Yanagimachi, R. Evidence suggesting that L-Fucose is part of a recognition signal for sperm zona pellucida attachment in mammals. Gamete Res 1982; 5: 355–61.Google Scholar

Familiari, G, Nottola, SA, Micara, G, Aragona, C, Motta, PM. Is the sperm-binding capability of the zona pellucida limited to its surface structure? A scanning electron microscopic study of human IVF. J In Vitro Fert Embry Trans 1988; 5: 134–8.CrossRef Google Scholar

Tesarik, J, Kopecny, V. Late preovulatory synthesis of proteoglycans by the human oocyte and cumulus cells and their secretion into the oocyte-cumulus-complex extracellular matrices. Histochem J 1986; 85: 523–8.Google Scholar PubMed

Overstreet, JW, Hembree, WC. Penetration of zona pellucida of non-living human oocytes by human spermatozoa in vitro. Fertil Steril 1976; 27: 815–31.CrossRef Google Scholar

Burkman, LJ, Coddington, CC, Franken, DR. The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 1988; 49: 688–97.Google Scholar

Liu, DY, Lopata, A, Baker, HWG. Use of oocytes that failed to be fertilized in vitro to study human sperm-oocyte interactions: comparison of sperm-oolemma and sperm-zona pellucida binding, and relationship with results of IVF. Reprod Fertil Dev 1990; 2: 641–50.Google Scholar

Franken, DR, Burkman, LJ, Oehninger, SC, Veeck, L, Kruger, TF, Coddington, CC, Hodgen, GD. The hemizona assay using salt stored human oocytes: evaluation of zona pellucida capacity for binding human spermatozoa. Gam Res 1989; 22: 15–26.Google Scholar

Sigman, M, Baazeem, A, Zini, A. Semen analysis and sperm function assays: what do they mean? Semin Reprod Med 2009; 27: 115–23.CrossRef Google Scholar PubMed

Janssen, M, Ombelet, W, Cox, A, Pollet, H, Franken, DR, Bosmans, S. The hemizona assay: a simplified technique. Arc of Andro 1997; 38: 127–31.Google Scholar

Vasan, SS. Semen analysis and sperm function tests: how much to test? Indian J Urol 2011; 27: 41–8.CrossRef Google Scholar PubMed

Franken, DR, Kruger, TF, Oehninger, SC, Kaskar, K, Hodgen, GD. Sperm binding capacity of human zona pellucida derived from oocytes obtained from different sources. Andrologia 1994; 26: 277–81.Google Scholar

Oehninger, SC, Franken, DR, Elyed, S, Barosso, G, Kolm, P. Sperm function and their predictive value for fertilization in IVF therapy: a meta analysis. Hum Reprod Update 2000; 6: 160–8.CrossRef Google Scholar PubMed

Aitken, RJ. Sperm function tests and fertility. Int J Aandrol 2006; 29: 69–75.CrossRef Google Scholar PubMed

Arslan, M, Morshedi, M, Arslan, EO, Taylor, S, Kanik, A, Duran, HF, Oehninger, S. Predictive value of the hemizona assay for pregnancy outcome in patients undergoing controlled ovarian hyperstimulation with intrauterine insemination. Fertil Steril 2006; 85: 1697–707.Google Scholar

Crosignani, PG, Rubin, BL. Optimal use of infertility diagnostic tests and treatments. Hum Reprod 2000; 15: 723–32.Google Scholar

Vogiatzi, P, Chrelias, C, Cahill, DJ, Creatsa, M, Vrachnis, N, Iliodromiti, Z, Kassanos, D, Siristatidis, C. Hemizona assay and sperm penetration assay in the prediction of IVF outcome: a systematic review. Biomed Res Int 2013: 945825.CrossRef Google Scholar

Samplaski, MK, Agarwal, A, Sharma, S, Sabanegh, E. New generation of diagnostic tests for infertility: review of specialized semen tests. Int J Urol 2010; 7: 839–47.Google Scholar

Hodgen, GD, Burkman, LJ, Coddington, CC, Franken, DR, Oehninger, S, Kruger, TF, Rosenwaks, Z. The hemizona assay (HZA): finding sperm that have the “right stuff”. J In Vitro Fert Embryo Transf 1988; 5: 311–13.CrossRef Google Scholar PubMed

Franken, DR, Kruger, TF, Lombard, CJ, Oehninger, SC, Acosta, AA, Hodgen, GD. The ability of the hemizona assay to predict human fertilisation in different and consecutive in-vitro fertilization cycles. Hum Reprod 1993; 8: 1240–4.CrossRef Google Scholar PubMed

Windt, ML, Franken, DR, Kruger, TF, Oehninger, SC. In vitro fertilization failure: identification of gamete defects by investigation of sperm-zona pellucida binding capacity of unfertilized oocytes. Andrologia 1996; 28: 211–15.Google Scholar

Oehninger, S, Morshedi, M, Franken, D. The Hemizona Assay for assessment of sperm function. Methods Mol Biol 2013; 927: 91–102.Google Scholar

Sanchez, R, Finkenzeller, C, Schill, WB, Miska, W. Comparison of two methods to obtain hemizonae pellucidae for sperm function tests. Hum Reprod 1995; 10: 2945–7.Google Scholar

Franken, DR, Oehninger, S. The clinical significance of sperm-zona pellucida binding: 17 years later. Front Biosci 2006; 11: 1227–33.CrossRef Google Scholar PubMed

Bleil, JD, Wasserman, PM. Structure and function of the zona pellucida: identification and characterization of the proteins of the mouse oocyte's zona pellucida. Dev Biol 1980; 76: 185–8.Google Scholar

Hammit, DG, Syrop, CH, Walker, DL, Bennet, MR. Conditions of oocyte storage and use of non-inseminated as compared with inseminated, non-fertilized oocytes for the hemizona assay. Fertil Steril 1993; 60: 131–6.Google Scholar

Morroll, DR, Lieberman, BA, Matson, PL. Use of human zonae from cryopreserved oocytes in a test to assess the binding capacity of human spermatozoa. IntJ Androl 1993; 16: 97–103.Google Scholar

Yoshimatsu, NR, Yanagimachi R, , Lopata, A. Zona pellucidae of salt-stored hamster and human eggs: their penetrability by homologous and heterologous spermatozoa. Gamete Res 1988; 21: 115–26.CrossRef Google Scholar PubMed

Henkel, R, Muller, C, Stalf, T, Schill, W-B, Franken, DR. Use of failed-fertilized oocyte for diagnostic zona binding purposes after sperm binding improvement with a modified medium. J Assist Reprod Genet 1999; 16: 24–9.Google Scholar

Henkel, R, Finkenzeller, C, Monsees, T, Franken, DR, Schill, W-B, Miska, W. Development of a new, highly sensitive zona pellucid binding assay using a bioluminescence-enhanced detection system. Andrologia 2001; 33: 215–21.Google Scholar

Romar R, Funahashi H, Coy, P. In vitro fertilization in pigs: new molecules and protocols to consider in the forthcoming years. Theriogenology 2016; 85: 125–34.CrossRef Google Scholar PubMed

Avella, MA, Baibakov, B, Dean, J. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol 2014; 205: 801–9.Google Scholar

Baibakov, B, Boggs, NA, Yauger, B, Baibakov, G, Dean, J. Human sperm bind to the N-terminal domain of ZP2 in humanized zonae pellucidae in transgenic mice. J Cell Biol 2012; 197: 897–905.CrossRef Google Scholar

Hamze, JG, Canha-Gouveia, A, Algarra, B, Gomez-Torres, MJ, Olivares, MC, Romar, R, Jimenez-Movilla, M. Mammalian spermatozoa and cumulus cells bind to a 3D model generated by recombinant zona pellucida protein-coated beads. Sci Rep 2019; 9: 179–89.CrossRef Google Scholar PubMed

Miranda, PV, Gonzalez-Echeverria, F, Marin-Briggiler, CI, Brandelli, A, Blaquier, JA, Tezon, JG. Glycosidic residues involved in human sperm–zona pellucida binding in vitro. Mol Human Reprod 1997; 3: 399–404.Google Scholar