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Chapter 9 - Capacitation and Acrosome Reaction: Fluorescence Techniques to Determine Acrosome Reaction

Published online by Cambridge University Press:  05 April 2021

By
Raúl Sánchez ,
Fabiola Zambrano  and
Pamela Uribe
Edited by
Ashok Agarwal ,
Ralf Henkel  and
Ahmad Majzoub
Ashok Agarwal
Affiliation:
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel
Affiliation:
University of the Western Cape, South Africa
Ahmad Majzoub
Affiliation:
Hamad Medical Corporation, Doha
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Summary

The acrosome reaction (AR) is an essential process of spermatozoa for fertilization and is characterized by the exocytosis of the acrosomal content and the release of hybrid membrane vesicles formed by patches of the outer acrosomal membrane and the plasma membrane. Through acomplex membrane fusion process, which exposes the inner acrosomal membrane, both the morphology of the plasma membrane and the distribution of proteins involved in sperm–egg interaction are changed [1, 2]. AR also modifies sperm function, since acrosome-reacted sperm are no longer able to bind to the zona pellucida, but to the oolemma [3].

Type
Chapter
Information
Manual of Sperm Function Testing in Human Assisted Reproduction , pp. 72 - 80
Publisher: Cambridge University Press
Print publication year: 2021

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