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Chapter 4 - Rescue ICSI of IVF Failed-Fertilized Oocytes

Published online by Cambridge University Press:  02 December 2021

By
Carly Barber ,
Vivian Kimble ,
Alexandrea Ramsey  and
Zsolt Peter Nagy
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
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Summary

Conventional IVF insemination has been providing satisfactory results in most cases in absence of male-factor infertility. However, low fertilization or complete fertilization failure can happen not only when sperm parameters are impaired but occasionally also when there is no detected male factor. In these occasions, to rescue the IVF treatment cycle, ICSI re-insemination of the failed-fertilized oocytes (after the original conventional insemination) may be attempted. Oocyte aging plays an important role, as because fertilization check is usually performed 16–18 hours after conventional insemination, when failure of fertilization can be detected, however, by that time outcomes after rescue ICSI is mostly suboptimal, if attempted. Alternatively, conventionally inseminated oocytes can be examined much sooner to detect signs of fertilization. It has been demonstrated, if oocytes are submitted to rescue ICSI within 4–8 hours after the initial insemination, then laboratory and clinical outcomes are significantly improved. Since conventional IVF insemination remains to be used frequently around the world, adequately performed rescue ICSI is a viable option to save IVF treatment cycles when unexpected low or total fertilization failure occur.

Keywords

Conventional inseminationFailed FertilizationFertilizationICSIImplantationIVFPregnancy
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 38 - 48
Publisher: Cambridge University Press
Print publication year: 2021

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References

Steptoe, PC, Edwards, RG. Birth after the reimplantation of a human embryo. Lancet 1978;2:366. https://doi.org/10.1016/s0140-6736(78)92957-4.CrossRefGoogle ScholarPubMed
Palermo, G, Joris, H, Devroey, P, Lancet, AVS. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17–8. https://doi.org/10.1016/0140–6736(92)92425-F.CrossRefGoogle ScholarPubMed
Yuzpe, AA, Liu, Z, Fluker, MR. Rescue intracytoplasmic sperm injection (ICSI) – salvaging in vitro fertilization (IVF) cycles after total or near-total fertilization failure. Fertil Steril 2000;73:1115–9. https://doi.org/10.1016/S0015-0282(00)00522-7.Google Scholar
Eftekhar, M, Pourmasumi, S, Razi, M-H. Efficacy of rescue ICSI after total fertilization failure in conventional IVF. J Infertil Reprod Biol 2013;1:58–62.Google Scholar
Mahutte, NG, Arici, A, Williams, L. Failed fertilization: is it predictable? Curr Opin Obstet Gynecol 2003;15:211–8. https://doi.org/10.1097/01.gco.0000072858.73466.aa.Google Scholar
Chen, L, Xu, Z, Zhang, N, Wang, B, Chen, H, Wang, S, et al. Neonatal outcome of early rescue ICSI and ICSI with ejaculated sperm. J Assist Reprod Genet 2014;31:823–8. https://doi.org/10.1007/s10815-014-0245-9.CrossRefGoogle ScholarPubMed
Chen, C, Kattera, S. Rescue ICSI of oocytes that failed to extrude the second polar body 6 h post-insemination in conventional IVF. Hum Reprod 2003;18:2118–21. https://doi.org/10.1093/humrep/deg325.Google Scholar
Nagy, ZP, Liu, J, Joris, H, Verheyen, G, Tournaye, H, Camus, M, et al. Andrology: the result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod 1995;10:1123–9. https://doi.org/10.1093/oxfordjournals.humrep.a136104.CrossRefGoogle Scholar
Nagy, ZP, Joris, H, Liu, J, Staessen, C, Devroey, P, Van Steirteghem, AC. Fertilization and early embryology: Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes. Hum Reprod 1993;8:2180–4. https://doi.org/10.1093/oxfordjournals.humrep.a138000.CrossRefGoogle Scholar
Lundin, K, Sjögren, A, Hamberger, L. Reinsemination of one-day-old oocytes by use of intracytoplasmic sperm injection. Fertil Steril 1996;66:118–21. https://doi.org/10.1016/s0015-0282(16)58397-6.Google Scholar
Swain, JE, Pool, TB. ART failure: oocyte contributions to unsuccessful fertilization. Hum Reprod Update 2008;14:431–46. https://doi.org/10.1093/humupd/dmn025.CrossRefGoogle ScholarPubMed
Trounson, A, Webb, J. Fertilization of human oocytes following reinsemination in vitro. Fertil Steril 1984;41:816–9. https://doi.org/10.1016/S0015-0282(16)47891-X.Google Scholar
Tesarik, J. Rescue ICSI revisited. Hum Reprod 2003;18:2122–3. https://doi.org/10.1093/humrep/deg425.CrossRefGoogle ScholarPubMed
Yuzpe, AA. The ART Laboratory. n.d.Google Scholar
Huang, B, Qian, K, Li, Z, Yue, J, Yang, W, Zhu, G, et al. Neonatal outcomes after early rescue intracytoplasmic sperm injection: an analysis of a 5-year period. Fertil Steril 2015;103:1432–1437.e1. https://doi.org/10.1016/j.fertnstert.2015.02.026.CrossRefGoogle ScholarPubMed
Zhu, L-x, Ren, X-l, Wu, L, Hu, J, Li, Y-f, Zhang, H-w, et al. Rescue ICSI: choose the optimal rescue window before oocyte aging. J Reprod Contracept 2011;22:29–36. https://doi.org/10.1016/S1001-7844(12)60004-2.Google Scholar
Liu, W, Liu, J, Zhang, X, Han, W, Xiong, S, Huang, G. Short co-incubation of gametes combined with early rescue ICSI: an optimal strategy for complete fertilization failure after IVF. Hum Fertil 2014;17:50–5. https://doi.org/10.3109/14647273.2013.859746.CrossRefGoogle ScholarPubMed
Nagy, ZP, Staessen, C, Liu, J, Joris, H, Devroey, P, Van Steirteghem, AC. Prospective, auto-controlled study on reinsemination of failed-fertilized oocytes by intracytoplasmic sperm injection. Fertil Steril 1995;64:1130–5. https://doi.org/10.1016/s0015-0282(16)57973-4.CrossRefGoogle Scholar
Jin, H, Shu, Y, Dai, S, Peng, Z, Shi, S, Sun, Y. The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen. Reprod Fertil Dev 2014;26:346–50. https://doi.org/10.1071/RD12369.Google Scholar
Nagy, ZP, Janssenswillen, C, Janssens, R, De Vos, A, Staessen, C, Van de Velde, H, et al. Timing of oocyte activation, pronucleus formation and cleavage in humans after intracytoplasmic sperm injection (ICSI) with testicular spermatozoa and after ICSI or in-vitro fertilization on sibling oocytes with ejaculated spermatozoa. Hum Reprod 1998;13:1606–12. https://doi.org/10.1093/humrep/13.6.1606.Google Scholar
Payne, D, Flaherty, SP, Barry, MF, Matthews, CD. Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod 1997;12:532–41. https://doi.org/10.1093/humrep/12.3.532.CrossRefGoogle ScholarPubMed
Van Den Bergh, M, Bertrand, E, Englert, Y. Second polar body extrusion is highly predictive for oocyte fertilization as soon as 3 hr after intracytoplasmic sperm injection (ICSI). J Assist Reprod Genet 1995;12:258–62. https://doi.org/10.1007/BF02212928.CrossRefGoogle Scholar
Moon, JH, Son, WY, Henderson, S, Mahfoudh, A, Dahan, M, Holzer, H. Spindle examination in unfertilized eggs using the polarization microscope can assist rescue ICSI. Reprod Biomed Online 2013;26:280–5. https://doi.org/10.1016/j.rbmo.2012.10.019.CrossRefGoogle ScholarPubMed
Nagy, ZP, Liu, J, Joris, H, Devroey, P, Van Steirteghem, A. Fertilization and early embryology: time-course of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection. Hum Reprod 1994;9:1743–8. https://doi.org/10.1093/oxfordjournals.humrep.a138786.Google Scholar
Cao, S, Wu, X, Zhao, C, Zhou, L, Zhang, J, Ling, X. Determining the need for rescue intracytoplasmic sperm injection in partial fertilisation failure during a conventional IVF cycle. Andrologia 2016;48:1138–44. https://doi.org/10.1111/and.12551.CrossRefGoogle ScholarPubMed
Hirabayashi, Y, Tawara, F, Ishii, M, Muramatsu, H, Kanayama, N. Evaluation of rescue ICSI on oocytes without extrusion of the second polar body in cases of moderately disturbed fertilization. J Mamm Ova Res 2006;23:135–40. https://doi.org/10.1274/jmor.23.135.Google Scholar
Chian, R-C, Lapensée, L, Phillips, S, Tan, S-L. Observation of pronuclei may not be an absolute indicator for fertilization in rescue intracytoplasmic sperm injection oocytes. Reprod Med Biol 2003;2:83–5. https://doi.org/10.1046/j.1445-5781.2003.00020.x.Google Scholar
Balakier, H. Fertilization and early embryology: tripronuclear human zygotes: The first cell cycle and subsequent development. Hum Reprod 1993;8:1892–7. https://doi.org/10.1093/oxfordjournals.humrep.a137955.Google Scholar
Jiaen, L, Zsolt, N, Hubert, J, Herman, T, Johan, S, Michel, C, et al. Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles. Hum Reprod 1995;10:2630–6. https://doi.org/10.1093/oxfordjournals.humrep.a135758.Google Scholar
Nagy, ZP, Rienzi, LF, Ubaldi, FM, Greco, E, Massey, JB, Kort, HI. Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection. Fertil Steril 2006;85:901–6. https://doi.org/10.1016/j.fertnstert.2005.09.029.Google Scholar
Gianaroli, L, Fiorentino, A, Magli, MC, Ferraretti, AP, Montanaro, N. Prolonged sperm-oocyte exposure and high sperm concentration affect human embryo viability and pregnancy rate. Hum Reprod 1996;11:2507–11. https://doi.org/10.1093/oxfordjournals.humrep.a019149.CrossRefGoogle ScholarPubMed
Kuczynski, W. Rescue ICSI of unfertilized oocytes after IVF. Hum Reprod 2002;17:2423–7. https://doi.org/10.1093/humrep/17.9.2423.Google Scholar
Jones, GM, Trounson, AO, Lolatgis, N, Wood, C. Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilization and embryo transfer. Fertil Steril 1998;70:1022–9. https://doi.org/10.1016/S0015-0282(98)00342-2.Google Scholar
Simopoulou, M, Giannelou, P, Bakas, P, Gkoles, L, Kalampokas, T, Pantos, K, et al. Making ICSI safer and more effective: a review of the human oocyte and ICSI practice. In Vivo 2016;30:387–400.Google Scholar
Tsirigotis, M, Nicholson, N, Taranissi, M, Bennett, V, Pelekanos, M, Craft, I. Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: diagnostic or therapeutic? Fertil Steril 1995;63:816–9. https://doi.org/10.1016/S0015-0282(16)57487-1.Google Scholar
Shalom-Paz, E, Alshalati, J, Shehata, F, Jimenez, L, Son, WY, Holzer, H, et al. Clinical and economic analysis of rescue intracytoplasmic sperm injection cycles. Gynecol Endocrinol 2011;27:993–6. https://doi.org/10.3109/09513590.2011.579655.Google Scholar
Jarmuz, P, Ocali, O, Baldwin, M, Sakkas, D, Barrett, C. Success rates of rescue ICSI and analysis of embryo cleavage rates by real time video imaging. Fertil Steril 2015;104:E301. https://doi.org/10.1016/j.fertnstert.2015.07.943.CrossRefGoogle Scholar
Ming, L, Liu, P, Qiao, J, Lian, Y, Zheng, X, Ren, X, et al. Synchronization between embryo development and endometrium is a contributing factor for rescue ICSI outcome. Reprod Biomed Online 2012;24:527–31. https://doi.org/10.1016/j.rbmo.2012.02.001.Google Scholar
Morton, PC, Wright, G, Yoder, CS, Brockman, WDW, Tucker, MJ, Kort, HI. Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure. Fertil Steril 1997;68:488–91. https://doi.org/10.1016/S0015-0282(97)00223-9.Google Scholar
Park, KS, Song, HB, Chun, SS. Late fertilization of unfertilized human oocytes in in vitro fertilization and intracytoplasmic sperm injection cycles: Conventional insemination versus ICSI. J Assist Reprod Genet 2000;17:419–24. https://doi.org/10.1023/A:1009409100941.Google Scholar
He, Y, Liu, H, Zheng, H, Li, L, Fu, X, Liu, J. Effect of early cumulus cells removal and early rescue ICSI on pregnancy outcomes in high-risk patients of fertilization failure. Gynecol Endocrinol 2018;34:689–93. https://doi.org/10.1080/09513590.2018.1433159.Google Scholar
Lombardi, E, Tiverón, M, Inza, R, Valcárcel, A, Young, E, Bisioli, C. Live birth and normal 1-year follow-up of a baby born after transfer of cryopreserved embryos from rescue intracytoplasmic sperm injection of 1-day-old oocytes. Fertil Steril 2003;80:646–8. https://doi.org/10.1016/S0015-0282(03)00996-8.CrossRefGoogle ScholarPubMed
Moon, JH, Henderson, S, Garcia-Cerrudo, E, Mahfoudh, A, Reinblatt, S, Son, WY. Successful live birth after transfer of blastocyst and frozen blastocyst from rescue ICSI with application of polarized light microscopy for spindle examination on unfertilized eggs. J Ovarian Res 2015;8. https://doi.org/10.1186/s13048-015-0150-6.Google Scholar
Esfandiari, N, Claessens, EA, Burjaq, H, Gotlieb, L, Casper, RF. Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes. Fertil Steril 2008;90:199.e5-199.e7. https://doi.org/10.1016/j.fertnstert.2007.07.1299.Google Scholar
Singh, N, Malhotra, N, Shende, U, Tiwari, A. Successful live birth after rescue ICSI following failed fertilization. J Hum Reprod Sci 2013;6:77–8. https://doi.org/10.4103/0974-1208.112388.Google Scholar
Teoh, PJ, Maheshwari, A. Low-cost in vitro fertilization: current insights. Int J Womens Health 2014;6:817–27. https://doi.org/10.2147/IJWH.S51288.Google Scholar
Xi, QS, Zhu, LX, Hu, J, Wu, L, Zhang, HW. Should few retrieved oocytes be as an indication for intracytoplasmic sperm injection? J Zhejiang Univ Sci B 2012;13:717–22. https://doi.org/10.1631/jzus.B1100370.Google Scholar
Sheehan, DA, Patel, JC, Pauli, SA, Pang, SC, Go, KJ. “Rescue” ICSI: saving a treatment cycle from fertilization failure. Fertil Steril 2013;100:S531. https://doi.org/10.1016/j.fertnstert.2013.07.290.Google Scholar
Pehlivan, T, Rubio, C, Ruiz, A, Navarro, J, Remohí, J, Pellicer, A, et al. Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes. J Assist Reprod Genet 2004;21:55–7. https://doi.org/10.1023/B:JARG.0000025939.26834.93.Google Scholar

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