Optimal Handling Techniques for Culture of Human Embryos

doi:10.1017/9781108874014.007

Chapter 6 - Optimal Handling Techniques for Culture of Human Embryos

Published online by Cambridge University Press: 15 April 2021

Michael L. Reed and

Thomas B. Pool

Edited by

Kersti Lundin and

Aisling Ahlström

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Kersti Lundin: Affiliation:
Sahlgrenska University Hospital, Gothenburg
Aisling Ahlström: Affiliation:
Livio Fertility Center, Gothenburg

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Summary

The purpose of this chapter is to review some of the factors that influence laboratory and clinical outcomes, broadly under the heading of optimal handling techniques. Two principal environments are encountered – inside and outside the incubator. The chapter will address media buffers, gas atmosphere, timing and setting up culture or holding vessels, protection of medium performance, temperature relative to handling gametes and embryos, lighting, pH, incubation choices, and workflow. The importance of the interplay between these variables cannot be overlooked.

Type: Chapter
Information: Manual of Embryo Culture in Human Assisted Reproduction , pp. 53 - 60

DOI: https://doi.org/10.1017/9781108874014.007 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2021

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