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2 - Pathology and cytogenetics

from Part I - LYMPHOMA OVERVIEW

Published online by Cambridge University Press:  05 March 2010

By
Andrew Wotherspoon ,
German Ott ,
Eugenia Haralambieva  and
Andreas Rosenwald
Edited by
Robert Marcus ,
John W. Sweetenham  and
Michael E. Williams
Andrew Wotherspoon
Affiliation:
Department of Histopathology, Royal Marsden Hospital, Fulham Road, London, SW3 6JJ, UK
German Ott
Affiliation:
Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2 Würzburg, 97080, Germany
Eugenia Haralambieva
Affiliation:
Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany
Andreas Rosenwald
Affiliation:
Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany
Robert Marcus
Affiliation:
Addenbrooke's NHS Foundation Trust, Cambridge
John W. Sweetenham
Affiliation:
Case Western Reserve University, Ohio
Michael E. Williams
Affiliation:
University of Virginia
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Summary

PREPARATION OF SPECIMENS FOR HISTOLOGY AND CYTOLOGY

The diagnosis of hematolymphoid tumors requires a combination of morphological, immunophenotypic, molecular genetic and clinical data. Successful morphological analysis is easiest to achieve by examination of intact lymph nodes that have been excised whole with the capsule intact. Fragmentation, while difficult to avoid in some areas (such as the mediastinum), inhibits analysis. Core biopsies are increasingly used for the diagnosis of lymphoid-related conditions, but these should be restricted to cases in which nodal excision is difficult or impossible, due either to access-related problems (intra-abdominal location) or to patient-related factors. Core biopsies, by their very nature, represent a small percentage of the lesional tissue and may fail to demonstrate focal variations in the tumor such as a diffuse component in follicular lymphoma. Fine needle aspiration (FNA) cytology has a role to play in the diagnosis of lymphoid pathology, but is best performed where there is access to high-quality flow cytometry that allows immunophenotyping of the lymphoid cells. FNA is particularly useful in the head and neck region for the exclusion of metastatic tumour.

Choice of the lymph node for sampling is of great importance. Where there is choice of areas the inguinal region is best avoided, as these nodes frequently show distortion of the normal architecture by fibrosis. Cervical lymph nodes are most easily interpreted.

Type
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Information
Lymphoma: Pathology, Diagnosis and Treatment , pp. 12 - 18
Publisher: Cambridge University Press
Print publication year: 2007

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References

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  • Pathology and cytogenetics
    • By Andrew Wotherspoon, Department of Histopathology, Royal Marsden Hospital, Fulham Road, London, SW3 6JJ, UK, German Ott, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2 Würzburg, 97080, Germany, Eugenia Haralambieva, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany, Andreas Rosenwald, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany
  • Edited by Robert Marcus, John W. Sweetenham, Case Western Reserve University, Ohio, Michael E. Williams, University of Virginia
  • Book: Lymphoma: Pathology, Diagnosis and Treatment
  • Online publication: 05 March 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511663369.003
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  • Pathology and cytogenetics
    • By Andrew Wotherspoon, Department of Histopathology, Royal Marsden Hospital, Fulham Road, London, SW3 6JJ, UK, German Ott, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2 Würzburg, 97080, Germany, Eugenia Haralambieva, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany, Andreas Rosenwald, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany
  • Edited by Robert Marcus, John W. Sweetenham, Case Western Reserve University, Ohio, Michael E. Williams, University of Virginia
  • Book: Lymphoma: Pathology, Diagnosis and Treatment
  • Online publication: 05 March 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511663369.003
Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

  • Pathology and cytogenetics
    • By Andrew Wotherspoon, Department of Histopathology, Royal Marsden Hospital, Fulham Road, London, SW3 6JJ, UK, German Ott, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2 Würzburg, 97080, Germany, Eugenia Haralambieva, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany, Andreas Rosenwald, Institute of Pathology, University of Würzburg, Josef-Schneider-Str, 2, Würzburg, 97080, Germany
  • Edited by Robert Marcus, John W. Sweetenham, Case Western Reserve University, Ohio, Michael E. Williams, University of Virginia
  • Book: Lymphoma: Pathology, Diagnosis and Treatment
  • Online publication: 05 March 2010
  • Chapter DOI: https://doi.org/10.1017/CBO9780511663369.003
Available formats
×