Book contents
- Frontmatter
- Contents
- List of contributors
- Preface
- Introduction
- SECTION I GRAFTINGS
- SECTION II SPECIFIC CHEMICAL REAGENTS
- SECTION III BEAD IMPLANTATION
- SECTION IV NUCLEIC ACID INJECTIONS
- SECTION V GENETIC ANALYSIS
- SECTION VI CLONAL ANALYSIS
- SECTION VII IN SITU HYBRIDIZATION
- SECTION VIII TRANSGENIC ORGANISMS
- SECTION IX VERTEBRATE CLONING
- SECTION X CELL CULTURE
- SECTION XI EVO–DEVO STUDIES
- SECTION XII COMPUTATIONAL MODELLING
- Appendix 1 Abbreviations
- Appendix 2 Suppliers
- Index
- Plate Section
Introduction
Published online by Cambridge University Press: 11 August 2009
- Frontmatter
- Contents
- List of contributors
- Preface
- Introduction
- SECTION I GRAFTINGS
- SECTION II SPECIFIC CHEMICAL REAGENTS
- SECTION III BEAD IMPLANTATION
- SECTION IV NUCLEIC ACID INJECTIONS
- SECTION V GENETIC ANALYSIS
- SECTION VI CLONAL ANALYSIS
- SECTION VII IN SITU HYBRIDIZATION
- SECTION VIII TRANSGENIC ORGANISMS
- SECTION IX VERTEBRATE CLONING
- SECTION X CELL CULTURE
- SECTION XI EVO–DEVO STUDIES
- SECTION XII COMPUTATIONAL MODELLING
- Appendix 1 Abbreviations
- Appendix 2 Suppliers
- Index
- Plate Section
Summary
Experiencing the process of scientific discovery is part of training to be a scientist. This book of laboratory exercises is designed to give students an opportunity to explore and carry out experiments that have each made significant contributions to the fields of Experimental Embryology and Developmental Biology over the past 100 years. It is our hope that students will experience the initial thrill of discovery as they learn how to do each experiment, analyze each outcome, and grasp the significance of each conclusion. However, science is not solely about the end discovery but also about the process. This process cannot be appreciated by reading textbooks or scientific journals alone. Rather, a budding scientist must experience first hand the myriad pitfalls of each experiment. Despite the way this laboratory manual is designed (with step-by-step instructions to accomplish each experiment), students will encounter unforeseen problems in carrying out the experiments. If they are not already intimately familiar with experimental science, students will undoubtedly discover that this process demands a meticulous approach. Designing, setting up, and executing experiments cannot be accomplished in a haphazard way. For this reason, every student must keep a laboratory notebook, a task that many initially regard as “busy work.” In fact, keeping careful record of everything one does in the laboratory is the only way to experience success. At the other end of this process is presenting a finished piece of work to the scientific community.
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- Publisher: Cambridge University PressPrint publication year: 2005