The chapter explores the role of Italian opera in the Brazilian Amazon during the Belle Époque and its effects on national and transnational identities. It focuses on the region’s most famous opera house – the Teatro Amazonas – and on the successes and misfortunes of the travelling companies that performed there between 1897 and 1907. The chapter probes the extent to which the opera house was considered a means of engaging with a ‘global fantasy of civilisation’, foregrounding the effects that local tropical diseases had on opera production and on global perceptions of the region during a period of keen interest in its commercial exploration. The shift from the Italian to the French repertoire at the start of the twentieth century sheds new light on Amazonian understandings of different notions of italianità, of Europe and of civilisation.