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Chapter 1 - Introduction to Immunohistochemistry

Published online by Cambridge University Press:  04 November 2017

Mai P. Hoang
Affiliation:
Harvard Medical School, Boston
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Publisher: Cambridge University Press
Print publication year: 2017

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References

Coons, AH, Creech, HJ, Jones, RN. Immunological properties of an antibody containing fluorescent group. Pro Soc Exp Biol Med 1941;47:200–2.Google Scholar
Nakane, PK. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: A study on pituitary glands of the rat. J Histochem Cytochem 1968;16:557–60.Google Scholar
Kohler, G, Milstein, C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975;256(5517):495–97.Google Scholar
Shi, SR, Key, ME, Kalra, KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39(6):741–48.Google Scholar
Hsu, SM, Raine, L, Fanger, H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 1981;75(5):734–38.Google Scholar
Moreau, A, Le Neel, T, Joubert, M, Truchaud, A, Laboisse, C. Approach to automation in immunohistochemistry. Clin Chim Acta 1998;278(2):177–84.Google Scholar
Feldman, MD. Beyond morphology: Whole slide imaging, computer-aided detection, and other techniques. Arch Pathol Lab Med 2008;132(5):758–63.CrossRefGoogle ScholarPubMed
Howat, WJ, Wilson, BA. Tissue fixation and the effect of molecular fixatives on downstream staining procedures. Methods 2014;70(1):1219.Google Scholar
O’Hurley, G, Sjostedt, E, Rahman, A, et al. Garbage in, garbage out: A critical evaluation of strategies used for validation of immunohistochemical biomarkers. Mol Oncol 2014;8(4):783–98.Google Scholar
Lipman, NS, Jackson, LR, Trudel, LJ, et al. Monoclonal versus polyclonal antibodies: Distinguishing characteristics, applications, and information resources. Inst Lab Anim Res J 2005;46(3):258–68.Google Scholar
Janeway, CA, Travers, P, Walport, M, Shlomchik, MJ. Chapter 3: Antigen recognition by B-cell and T-cell receptors. Immunobiology, 5th edition. New York: Garland Science,, 2001.Google Scholar
Bogen, SA, Vani, K, Sompuram, SR. Molecular mechanisms of antigen retrieval: Antigen retrieval reverses steric interference caused by formalin-induced cross-links. Biotech Histochem 2009;84(5):207–15.CrossRefGoogle ScholarPubMed
Sompuram, SR, Vani, K, Hafer, LJ, et al. Antibodies immunoreactive with formalin-fixed tissue antigens recognize linear protein epitopes. Am J Clin Pathol 2006;125(1):8290.Google Scholar
Schofield, DJ, Lewis, AR, Austin, MJ. Genetic methods of antibody generation and their use in immunohistochemistry. Methods 2014;70(1):2027.Google Scholar
Khoury, T, Sait, S, Hwang, H, et al. Delay to formalin fixation effect on breast biomarkers. Mod Pathol 2009;22(11):1457–67.Google Scholar
Goldstein, NS, Hewitt, SM, Taylor, CR, et al. Recommendations for improved standardization of immunohistochemistry. Appl immunohistochem Mol Morphol 2007;15(2):124–33.CrossRefGoogle ScholarPubMed
Xie, R, Chung, J-Y, Ylaya, K, et al. Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. J Histochem Cytochem 2011;59(4):356–65.CrossRefGoogle ScholarPubMed
Warford, A, Akbar, H, Riberio, D. Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems. Methods 2014;70(1):2833.Google Scholar
Brandtzaeg, P. The increasing power of immunohistochemistry and immunocytochemistry. J Immunol Methods 1998;216(1–2):4967.Google Scholar
Pitts, A, Williams, EE. Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies. J Histochem Cytochem 1974;22(1):51–4.Google Scholar
Brown, KJ, Pemberton, AD, Wright, SH, Miller, HRP. Primary antibody-Fab fragment complexes: A flexible alternative to traditional direct and indirect immunolabeling techniques. J Histochem Cytochem 2004;52(9):1219–30.Google Scholar
Mason, TE, Phifer, RF, Spicer, SS, Swallow, RA, Dreskin, RB. An immunoglobulin enzyme bridge method for localizing tissue antigens. J Histochem Cytochem 1969;17(9):563–69.CrossRefGoogle ScholarPubMed
Sternberger, LA, Hardy, PH, Cuculis, JJ, et al. The unlabeled antibody enzyme method of immunohistochemistry: Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 1970;18(5):315–33.Google Scholar
Hsu, S-M, Raine, L, Fanger, H. Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29(4):577–80.Google Scholar
Elias, JM, Margiotta, M, Gaborc, D. Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP), avidin-biotin peroxidase complex (ABC), and peroxidase-labeled avidin-biotin (LAB) methods. Am J Clin Pathol 1989;92(1):6267.Google Scholar
Cordell, JL, Falini, B, Erber, WN, et al. Immunoenzymatic label of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32(2):219–29.Google Scholar
Wagner, L, Worman, CP. Color-contrast staining of two different lymphocyte subpopulations: A two-color modification of alkaline phosphatase monoclonal anti-alkaline phosphatase complex technique. Stain Technol 1988;63(3):129–36.Google Scholar
Stack, EC, Wang, C, Roman, KA, Hoyt, CC. Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods 2014;70(1):4658.Google Scholar
Sano, T, Smith, CL, Cantor, CR. Immuno-PCR: Very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 1992;258(5079):120–22.Google Scholar
Chen, B-X, Szabolcs, MJ, Matsushima, AY, Erlanger, BF. A strategy for immunohistochemical signal enhancement by end-product amplification. J Histochem Cytochem 1996;44(8):819–24.Google Scholar
Wiedorn, KH, Goldmann, T, Henne, C, Kuhl, H, Vollmer, E. EnVision+, a new dextran polymer-based signal enhancement technique for in situ hybridization (ISH). J Histochem Cytochem 2001;49(9):1067–71.Google Scholar
Engel, KB, Moore, HM. Effects of preanalytical variable on the detection of proteins by immunohistochemistry in formalin-fixed, paraffin-embedded tissue. Arch Pathol Lab Med 2011;135(5):537–43.Google Scholar
Fetsch, PA, Abati, A. The clinical immunohistochemistry laboratory: Regulations and troubleshooting guidelines. Methods Mol Biol 2010;588:399412.CrossRefGoogle ScholarPubMed
Fitzgibbons, PL, Bradley, LA, Fatheree, LA, et al. Principles of analytic validation of immunohistochemical assays. Guideline from the College of American Pathologists Pathology and Laboratory Quality Center. Arch Pathol Lab Med 2014;138(11):1432–43.Google Scholar
Kothmaier, H, Rohrer, D, Stacher, E, et al. Comparison of formalin-free tissue fixative. A proteomic study testing their application for routine pathology and research. Arch Pathol Lab Med 2011;135(6):744–52.Google Scholar
Jin, Z, Hildebrandt, N. Semiconductor quantum dots for in vitro diagnostics and cellular imaging. Trends Biotech 2012;30(7):394403.Google Scholar
Chen, C, Sun, SR, Gong, YP, et al. Quantum dots-based molecular classification of breast cancer by quantitative spectroanalysis of hormone receptors and HER2. Biomaterials 2011;32(30):7592–99.Google Scholar
Tubbs, RR, Wang, H, Wang, Z, et al. Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. Am J Clin Pathol 2013;140(5):736–46.Google Scholar
Ricciuti, B, Mencaroni, C, Paglialunga, L, et al. Long noncoding RNAs: New insights into non-small cell lung cancer biology, diagnosis and therapy. Med Oncol 2016;33(2):10. doi: 10.1007/s12032-016–0731-2.CrossRefGoogle ScholarPubMed

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