Molecular Sensors for Transcriptional and Post-Transcriptional Assays

doi:10.1017/CBO9781139021500.018

Chapter 13 - Molecular Sensors for Transcriptional and Post-Transcriptional Assays

from Section Four - Chemical Genomics Assays and Screens

Published online by Cambridge University Press: 05 June 2012

Douglas S. Auld and

Natasha Thorne

Edited by

Haian Fu

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Haian Fu: Affiliation:
Emory University, Atlanta

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Summary

Cell-based assays allow researchers to probe the regulation of cell signaling, communication, and regulation within a system that is more physiologically relevant than assays that focus on isolated molecular components or cell extracts (biochemical assays). The application of cell-based assays in high-throughput screening (HTS) has increased in recent years. It is estimated that cell-based assays comprise more than half of HTS assays used in pharmaceutical industries [1].

Cell-based molecular sensor assays have been developed to measure either transcriptional or post-transcriptional events (Figure 13.1). A typical transcriptional reporter gene assay involves the placement of a cis-regulatory element in front of a gene that produces a measurable signal in response to modulation of transcriptional complexes that are regulated by ligands or complex networks. Assays favored in HTS are usually constructed to generate fluorescence or luminescence by expressing either an enzyme or a fluorescent protein reporter. Post-transcriptional assays have been developed by engineering the reporter to respond to events such as mRNA splicing, protein complex formation, translocation of a receptor between cellular compartments, or protein stability.

Type: Chapter
Information: Chemical Genomics , pp. 173 - 197

DOI: https://doi.org/10.1017/CBO9781139021500.018 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2012

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