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19 - Measuring Hormones: Considerations for Biospecimen Collection, Assay, and Analysis

from Part V - Physiological Measures

Published online by Cambridge University Press:  12 December 2024

By
Shannin N. Moody ,
Amali I. Stephens ,
Jenny Mai Phan ,
Olga Miocevic ,
Amita Kapoor ,
Wen Wang ,
Allissa L. Van Steenis ,
Scott Le ,
Lotte van Dammen  and
Elizabeth A. Shirtcliff
Edited by
John E. Edlund  and
Austin Lee Nichols
John E. Edlund
Affiliation:
Rochester Institute of Technology, New York
Austin Lee Nichols
Affiliation:
Central European University, Vienna
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Summary

This chapter presents a broad overview of the measurement of hormones, spanning from their collection in different biospecimens and the assay of hormones across laboratory strategies to a brief overview of statistical treatment and analysis that extracts the hormone of interest. We organize each section into a description of measurement tools followed by an agnostic analysis of the tools for their strengths, weaknesses, prospects, and pitfalls. We do not view any single approach as “best” or “optimal.” This view is commensurate with the production and cellular conversion of hormones – adaptive physiological processes that are not “best” or “optimal” but rather constantly changing biobehavioral markers that shift according to the demands of the environment. Measuring the hormone is just the beginning of exploring the multifaceted ways that hormones can inform health, development, morbidity, and mortality.

Type
Chapter
Information
The Cambridge Handbook of Research Methods and Statistics for the Social and Behavioral Sciences
Volume 2: Performing Research
 , pp. 427 - 454
Publisher: Cambridge University Press
Print publication year: 2024

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