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Chapter 10 - Mouse Embryo Assay for Quality Control in the IVF Laboratory

from Section 2 - Pre-procedure Protocols

Published online by Cambridge University Press:  07 August 2023

By
Dean E. Morbeck
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Ensuring proper quality control (QC) in the laboratory is critical to the success of any in vitro fertilization (IVF) programme, as the environment of the laboratory can alter the quality of the embryos produced. The ultimate role of the embryology laboratory is to maintain the inherent viability of the gametes and embryos in an environment outside the female reproductive tract. There is a need for objective, sensitive and reproducible methods and assays for testing materials for embryo toxicity as well as growth promoting and inhibiting factors. The manufacturers test commercially available IVF media and plastic ware and provide the results of their testing with the shipment of supplies. It may be advisable to test media and supplies upon arrival to confirm that nothing occurred during shipping. The suitability of various reagents and materials for use in human IVF can be tested using a range of bioassays. While the most used bioassays for QC in IVF laboratories are the human sperm survival assay and the mouse embryo bioassay (MEA), the one-cell MEA has been consistently shown to be the most sensitive.

Keywords

quality controlmouse embryotoxicity
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 75 - 79
Publisher: Cambridge University Press
Print publication year: 2023

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References

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