Sulfur cinquefoil is an invasive, perennial forb on rangelands of western Canada and the United States. Sulfur cinquefoil reproduces solely by seed and it is a prolific seed producer. Our 2-yr study (2006, 2007) investigated the optimal timing and intensity of defoliation to decrease aboveground productivity and seed production of sulfur cinquefoil plants on foothill rangeland in southwestern Montana. Each year, 150 sulfur cinquefoil plants within a 430-m2 enclosure were tagged for identification and randomly assigned to one of 15 clipping treatments with 10 plants per treatment. Clipping treatments were conducted at three timings: (preflower [early June], flowering [late June], and seedset [mid-July]) and all possible combinations of timings for a total of seven timing treatments clipped to two stubble heights (7.5 cm or 15 cm), comprising 14 unique treatments. The final (15th) treatment consisted of an unclipped control. Response variables collected at senescence (late July) included aboveground biomass; number of buds, flowers and fruits on each plant; and number and viability of seeds produced. Results indicated that defoliation of sulfur cinquefoil can effectively reduce its yield and seed production. All clipping treatments reduced aboveground biomass of sulfur cinquefoil compared with control plants (P ≤ 0.05), except clipping to 15 cm during preflowering in the wetter year of 2006. Clipping to either 7.5 cm or 15 cm at all times or combinations of timings reduced the number of buds, flowers, fruits, and seeds produced by sulfur cinquefoil (P ≤ 0.05). Viable seed production was reduced 99 to 100% when plants were clipped once to either 7.5 or 15 cm during flowering or seedset. Results suggest that targeted livestock grazing or mowing applied one time per season during flowering or seedset could effectively suppress the biomass production and viable seed production of sulfur cinquefoil.