The size of reptile hatchlings can be phenotypically plastic in response to incubation temperature, and size is a trait likely to influence fitness – i.e. hatchling size is proposed as an indicator of quality. The parental and incubation temperature effects on the size of one of New Zealand's most biologically significant reptile species, the tuatara Sphenodon punctatus are investigated. Artificial incubation at constant temperatures is used to produce founders for new captive and wild populations of tuatara and to augment existing rare populations. We compare size of hatchling tuatara from artificial and natural incubation treatments. The relationship of hatchling size with incubation temperature and sex is examined, and we investigate whether our results support differential fitness models for the evolution of temperature-dependent sex determination in tuatara. Initial egg mass is the most important factor affecting size of hatchling tuatara and is still an important influence at 10 months of age. Incubation temperature does not greatly influence size of hatchlings, but significantly influences size by 10 months of age. Constant artificial incubation conditions result in larger, but possibly less aggressive, juveniles than those from more variable natural incubation conditions by 10 months of age. Evidence from size patterns of tuatara incubated in natural nests supports differential fitness models for the adaptive significance of temperature-dependent sex determination. Thermal variation has little effect on size of male hatchlings, but female embryos that develop in more stable thermal conditions, in more reliable sites for hatching, are bigger and have longer jaws.

Northern tuatara Sphenodon punctatus punctatus survive on about 26 islands off the north-east coast of the North Island, New Zealand. These rare, lizard-like reptiles include seabirds in their diet. The presence of the Pacific rat Rattus exulans on some islands has been implicated in the decline of tuatara populations through predation and competition for food. Pacific rats and tuatara eat similar dietary items, including seabirds. Seabirds have high levels of the long chain n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The potential contribution of seabirds to the diet of tuatara was investigated by measuring the composition of plasma fatty acids and the concentrations of triacylglycerol and cholesterol of adult tuatara from Green Island (rat-free, numerous seabirds) and Coppermine Island (rat-inhabited, relatively few seabirds). The composition of plasma fatty acids differed between islands and sexes. Specifically, tuatara from rat-free Green Island had higher levels of the long chain PUFAs arachidonic acid, EPA and DHA compared to tuatara on rat-inhabited Coppermine Island, and males had significantly higher levels of DHA than females on both islands. Concentrations of plasma triacylglycerol and cholesterol were higher in tuatara from Green Island than Coppermine Island. Males had higher cholesterol but lower triacylglycerol concentrations than females. Differences in fatty acid composition and lipid concentrations between groups of tuatara imply differences in diet. Tuatara on the rat-free island evidently had a higher intake of n-3 PUFAs compared with tuatara on the rat-inhabited island. This is probably due to higher seabird consumption, or perhaps to the presence of other dietary items containing EPA and DHA. High concentrations of plasma triacylglycerol and cholesterol may be the result of a high intake of insect larvae. We recommend the direct investigation of the diets of northern tuatara and the consequences of differences in the composition of plasma fatty acids for reproduction.