Enhancing seedling quality and promoting root growth post-transplantation are crucial for improving mechanically transplanted rice productivity. Here we investigated the impact of various plant growth regulators on hybrid and conventional rice varieties. Treatments, including two-diethylaminoethyl hexanoate (DA-6, 10 mg L−1), a combination of potassium 3-indole-butyrate + potassium 1-naphthylacetate + 6-benzylaminopurine (C3, 50 + 50 + 10 mg L−1), potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1-triacontanol (C4, 50 + 50 + 50 mg L−1), potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1.8% sodium nitrophenolate (C5, 50 + 50 + 1 mg L−1), and a combination of potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1.8% sodium nitrophenolate + DA-6 (C6, 50 + 50 + 1 + 10 mg L−1), were sprayed either 3 or 10 days before transplanting. Seedlings sprayed 10 days before transplanting exhibited a higher number of white roots and total roots at the returning green stage, along with increased grain yield, irrespective of the plant growth regulator used. The C6 combination emerged as the most effective treatment, enhancing the growth of both hybrid and conventional rice seedlings, accelerating the growth rate of white roots and total roots, and increasing the length of the longest white root during the greening period, ultimately resulting in higher grain yield. Our findings demonstrate that pre-transplantation application of a combination of plant growth regulators positively influences rice seedling growth.

Modification of the existing cropping practice is needed to maintain rice (Oryza sativa L.) productivity and reduce irrigation water input. A 2-year field experiment was conducted during the dry rice growing season of 2016 and 2017 at the Asian Institute of Technology, Pathum Thani, Thailand, to investigate the effects of establishment method and irrigation level on growth, yield, and water productivity of irrigated lowland rice. The treatments consisted of two Thai rice cultivars (Pathumthani 1 and RD57), two establishment methods (dry direct seeding [DDS] and transplanting [TP]), and three irrigation levels (continuous flooding [CF], 15 cm threshold water level below the soil surface for irrigation [AWD15], and 30 cm threshold water level below the soil surface for irrigation [AWD30]). Overall, the performance of RD57 was better than Pathumthani 1 under DDS with 50% higher grain yield and 90% higher water productivity at AWD15. RD57 also had higher shoot dry matter, number of tiller m–2, and number of panicle m–2 across establishment methods and irrigation levels. Grain yield and water productivity of RD57 were similar under two establishment methods across irrigation levels, whereas the performance of TP was better than DDS for Pathumthani 1 irrespective of irrigation levels. The highest grain yield and water productivity of Pathumthani 1 was observed at AWD15 under TP and that of RD57 under both establishment methods at the same irrigation level. AWD15 saved 26 and 32% irrigation water under TP and DDS, respectively, compared with TP-CF treatment combination. AWD15 irrigation level could be recommended for greater water productivity without compromising yield when Pathumthani 1 is cultivated through TP and RD57 is cultivated through either DDS or TP. Although water-saving potential was higher compared with CF, AWD30 is not recommended for irrigated lowland rice cultivation due to significant yield reduction.

Introduction. Although several pineapple micropropagation protocols have already been published, significant improvement could be achieved if the stages of the in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Materials and methods. Axillary buds were inoculated on an MS liquid culture medium added with NAA (2 μM) and BAP (4 μM). Regenerated shoots, divided into three classes of different sizes, were then used in further experiments. First, these shoots were inoculated in flasks containing the same MS culture medium with or without growth regulators. Then, four basic media, containing different salts and free from growth regulators were tested. In a third assay, the MS culture medium was compared with a half-diluted MS culture medium for studying the plantlet elongation and rooting stage. Results. In the MS culture medium supplemented with NAA and BAP, the highest multiplication rate (13.5 shoots) was obtained with the smallest shoots inoculated, while in the MS culture medium free of growth regulators, the highest plantlets (7.7 cm) were the result of the highest shoots inoculated and showed no vitrification. The normal MS culture medium, in comparison with the half-diluted one and the three other salt formulations, revealed a significant increase in the plantlet elongation and best general features. For acclimatization, the highest values of the survival rate (93.8% ) and fresh and dry weights were obtained with the transference of higher than 7.0 cm in vitro plantlets. Conclusion. Using the protocol described in this work, it is possible to obtain 1 million in vitro plantlets after 9 months from a single bud, with a 45 day subculture interval and an average multiplication rate of 10 shoots per bud.