An inverse method for identifying the element rigidities of frame structures is developed in this paper. The primary input of this method is the longitudinal strains of the frame elements measured in transient tests. The spectral element method is employed to formulate the equilibrium equation of the frame in the frequency domain. The equilibrium equation is rewritten in terms of the strains of the frame elements. The identification problem is then formulated as an optimization program in which the error norm of the equilibrium equation is minimized. The proposed method is applicable to total structure identification as well as substructure identification. The issue of identifiability is addressed in this study. A numerical example is presented to illustrate the proposed method. The influence of noise is investigated through the example.