This article describes the important role of shepherdesses of the Tzotzil ethnic group from the mountains of Chiapas State, in southern México, not only in the historical rescue of several Spanish sheep breeds but also in the conservation of this species. Due to the legacy of their millenarian Mayan culture and the historical blending with elements of the Catholic religion during Colonial times, the Tzotzil women are responsible for caring for their sacred sheep that receive given names and special consideration. Women are also in charge of making all the woolen clothes that identify the members of their ethnic group. The typical garments are handmade with the fleeces obtained from the local sheep, processed by means of traditional textile techniques such as the spinning of threads with a spindle and the weaving using the back-strap loom of pre-Hispanic design. Clothes for daily and ceremonial use, both for Tzotzil men and women include heavy black coats and skirts, white woolen ponchos, brown blouses, black shawls, all of them made out of wool, and this has represented the need to preserve the diversity of sheep with different phenotypes and kept under a traditional management system.