Fourteen organic-rich sedimentary layers in the deposits at Quebrada de los Burros, in coastal southern Peru (Tacna department), lie between two debris-flow units, interpreted to result from El Niño events, at 8980 cal yr B.P. and after 3380 cal yr B.P., respectively. The accumulation of the fine-grained and low-energy sediments of this deposit during the mid-Holocene is incompatible with the occurrence of El Niño events in this region, as these would produce catastrophic flood deposits. The occurrence of organic-rich sediments and evidence of an enhancement of upwelling strength at this time imply the existence of a permanent water supply resulting from an increased condensation of fog at mid-altitudes. These results suggest a lower intensity and perhaps, a lower frequency of occurrence of the El Niño phenomenon during the mid-Holocene. It is precisely during this period that the most important human settlements are found at this site, probably indicating the presence of reliable supply of fresh water. The chronologies for wetlands in the central south altiplano are out of phase with those indicating increased soil moisture episodes on the coast, implying a long-term difference in climate between these two regions.