Dryland and irrigated field experiments were established to measure differences in soil water relations throughout the growing season between plots in which cotton was grown with and without silverleaf nightshade interference. Soil moisture readings were taken weekly at 15-cm increments to a maximum depth of 120 and 150 cm during 1984 and 1985, respectively. When cotton was grown with silverleaf nightshade, soil water loss was greater at the lower portion of the soil profile earlier in the growing season than when cotton was grown alone. In the irrigated environment in 1985 when precipitation was higher than normal and the soil moisture content remained above normal, soil water loss did not differ. Cotton lint yield, plant height, and boll size reflected the amount of soil moisture available for growth and development of the crop. A statistical technique for quantifying soil water relations between the crop growing alone and growing with interference from the weed is demonstrated.