Five experiments were conducted during 2001 and 2002 in North Carolina to evaluate peanut injury and pod yield when glyphosate was applied to 10 to 15 cm diameter peanut plants at rates ranging from 9 to 1,120 g ai/ha. Shikimic acid accumulation was determined in three of the five experiments. Visual foliar injury (necrosis and chlorosis) was noted 7 d after treatment (DAT) when glyphosate was applied at 18 g/ha or higher. Glyphosate at 280 g/ha or higher significantly injured the peanut plant and reduced pod yield. Shikimic acid accumulation was negatively correlated with visual injury and pod yield. The presence of shikimic acid can be detected using a leaf tissue assay, which is an effective diagnostic tool for determining exposure of peanut to glyphosate 7 DAT.