Dolphins in captivity have to cope with severe changes in their environment. So far, there are few studies on the welfare of these animals under these conditions. The aim of the present study was to find if cortisol was present in the saliva of dolphins and to explore the possibility of performing serial, non-invasive cortisol assays in captive dolphins. Saliva was collected non-invasively during a month from four dolphins that had responded to previous training, in order to provide saliva samples, in two aquaria in Mexico City. In addition, serum and saliva time-matched samples were obtained in an aquarium in Nuevo Vallarta, Mexico. Cortisol concentrations in saliva and blood were measured by radioimmunoanalysis (RIA). Results show for the first time that measurable quantities of cortisol are secreted within the saliva of dolphins. Salivary cortisol measurements could be a useful tool for carrying out long-term cortisol sampling. It is far less invasive than blood-sampling and could be used, in conjunction with behavioural observations, to monitor the welfare of captive dolphins, non-invasively.

PTSD among a sample of mass-evacuated adults from Kosovo was studied using a prospective design with a baseline study and follow-ups at 3 and 6 months in Sweden, and with an additional follow-up after 1.5 years in both Sweden and Kosovo. Trauma events and PTSD-related symptoms were measured by the Harvard Trauma Questionnaire (HTQ). At the additional follow-up after 1.5 years the same measure (HTQ) was used as well as clinical diagnostic interviews with the SCID instrument and measurement of saliva cortisol levels. Thirty-seven percent had PTSD-related symptoms at baseline. Morbidity increased at the three follow-ups. About 80% of the participants had PTSD at the additional follow-up after 1.5 years. The HTQ results were confirmed by clinical diagnoses and the participants diagnosed with PTSD also had low saliva cortisol levels. The results are discussed in terms of trauma, time needed to develop PTSD, post-migration stress and selection mechanisms.