Studies on losses in yield of rapeseed, Brassica campestris cv. Brown Sarson, caused by the mustard aphid, Lipaphis erysimi (Kalt.) were conducted under natural and artificial conditions. Based on the experiments on the influence of aphid exposure to the crop on aphid number and seed yield and the effect of varied aphid population at flowering/pod initiation stage and artificial release of aphids at 10-day intervals starting from 40 days of sowing on the plant height, number of siliquae per plant, number of primary branches, seed yield per plant and 1000-grain weight, a positive correlation between length of aphid exposure to the crop and aphid population was found. A negative relationship between aphid population, length of aphid exposure and seed yield was observed. An inverse and significant relationship existed between parameters contributing to yield loss and losses in seed yield. However, this relationship was not significant in siliquae per plant and 1000-grain weight. A similar relationship was observed in artificial infestation.