A study was conducted to compare the effects of ammoniation and protein supplementation of barley straw on rumination behaviour of sheep. Four wethers were allocated to four diets offered ad libitum in a 4 × 4 Latin-square design. The diets were, untreated barley straw + molasses meal (diet 1), untreated barley straw + soya-bean meal + molasses meal (diet 2), ammonia-treated barley straw + molasses meal (diet 3) and ammonia-treated barley straw + soya-bean meal + molasses meal (diet 4). Animals were kept in metabolism crates throughout each 16-day experimental period and allowed free access to water and a mineralized salt lick. The first 11 days of each period were for adaptation to the harnesses and diets while the last 5 days were used for rumination studies. Animals given diets 3 and 4 had slower eating rates compared with those given diets 1 and 2. Rumination index and duration of each rumination period was lower for sheep consuming diets 3 and 4 than for those on diets 1 and 2 but not significantly so (P > 0·05). Rumination time per 100 g neutral-detergent fibre (NDF) intake was significantly lower (P < 0·01) for diets containing ammoniated straw. Animals given ammoniated straw diets also regurgitated fewer boluses per unit NDF intake than did those on untreated straw diets. The results showed that increased intake and digestibility, which is usually associated with ammoniated straws, was due to sheep doing less work per unit of time to break down straw for digestion. In this way, potentially digestible tissues within a given amount of straw is more readily exposed. The foregoing suggests that ammonia treatment results in less rumination so that ruminants given ammonia-treated straw diets do less work ruminating.