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Chinese Journal of Agricultural Biotechnology (1)

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Construction of conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism
Chen Fen, Xiong Wei, Min Yong, Fan Yu-Qing, Liang Yun-Xiang, Lü He-Ping, Xing Ren-Chang, Zheng Ying-Hua
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 5 / Issue 1 / April 2008

Published online by Cambridge University Press:

27 June 2008, pp. 73-79

Print publication:

April 2008
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Intergeneric transfer of plasmid vectors pSET152 and pHL212 from donor Escherichia coli ET12567/pUZ8002 and S17-1 to Streptomyces cinnamonensis was demonstrated and optimized. Assisted by this conjugation system, nsdA gene disruption was achieved through PCR-targeted gene replacement. One AprRKanS exconjugant BIB309 was then isolated and confirmed to be the nsdA null mutant. Compared with the starting strain, monensin production by the nsdA− mutant BIB309 increased 270% in vitro.