Field tests were carried out to compare the performance of a new tsetse trap, the Nitse (I and II) with the biconical, F3 and NGU traps respectively at the Yankari Game Reserve, Bauchi State, Nigeria. The game reserve harbours Glossina palpalis, G. tachinoides and G. morsitans submorsitans species. The biconical trap was used as the standard. The sites had been cleared of vegetation; they were 5–10 m away from vehicle paths and about 100 m apart. Each trap was moved rotatingly until all traps were exposed to each of the five sites. Traps were emptied each morning after 24 h and the tsetse sorted according to species and sex. The data on the different trap catches were analysed using the one-way ANOVA and the multiple range test via the SPSS statistical package. The performance of the Nitse I on G. tachinoides in one area with high tsetse density was significantly better than that of each of the three traps. But for G. m. submorsitans, Nitse I and the biconical trap performed better than only the F3 (P ≤ 0.05) in the high density area. Only the biconical performed significantly better than the F3 trap in an area with low tsetse density. The new Nitse trap was more effective for G. tachinoides than for G. m. submorsitans.