The upper half of the Archerbeck Borehole contains a continuous Mississippian succession from the late Asbian (late Viséan) to the Pendleian (early Serpukhovian), with numerous limestone horizons. The borehole sequence lies within the Solway Basin (western end of the Northumberland Trough) and bridges the successions between the Midland Valley of Scotland and the Pennines (northern England). The rich foraminiferal and algal assemblages are compared to those described by previous authors, and genera as well as species are updated as far as is possible. In addition, some other overlooked or underestimated taxa have been identified and illustrated, which improve notably the biostratigraphic resolution of the Archerbeck Borehole succession. Within the abundant foraminiferal assemblages can be highlighted the richness and diversity of representatives of the family Archaediscidae. In general, the succession developed in the Archerbeck Borehole is closely comparable with that in the Alston Block of northern England. The Asbian/Brigantian transition mimics that observed in the Janny Wood boundary stratotype section, with clear late Asbian horizons (lower part of the Archerbeck Beds), transitional beds (middle and upper parts of the Archerbeck Beds), and Brigantian limestones (from the Cornet Limestone upwards). The base of the early Brigantian is placed at the base of the Cornet Limestone (equivalent to the Lower Peghorn Limestone in the Alston Block). The base of the late Brigantian is placed at the base of the Gastropod Limestone (equivalent to the Scar Limestone Member in northern England), and the base of the Pendleian is repositioned at the base of the Under Limestone (equivalent to the Four Fathom Limestone Member in northern England). Throughout, the borehole, protista and microfloral elements are abundant, which allows the recognition of Assemblages 4 to 10, previously recognised in northern England and the Midland Valley of Scotland. Furthermore, other local assemblages are recognised and related to palaeoecological controls, although they do not represent any improvement in biostratigraphical resolution.