An analytical and a numerical study on coupling in microstrip transmission line (MTL) are reported for different split-gap orientations of a single gap square-shaped split-ring resonator (SRR). Taking both magnetic and electric couplings and adopting Lagrangian formulation for this coupled system, mathematical relations are found for each orientation of SRR to determine the connection between coupling co-efficient and resonant mode frequency. It is shown that coupling results in higher resonance frequency when the SRR, with parallel split-gap, has the gap far from the MTL. But, a lower resonance frequency is obtained when the SRR is placed at shorter distances with parallel split-gap near to the MTL. Again, for perpendicular split-gap orientation of SRR, the resonant frequency is found in terms of an effective coupling co-efficient; at shorter distances it is found to be lower than the fundamental resonance frequency of uncoupled SRR and an opposite effect is obtained at longer distances. Using CST Microwave Studio, the resonance frequency for each split-gap orientation of SRR is also studied as a function of separation between MTL and SRR. It is observed that this phenomenon strongly depends on SRR side-length, substrate height, and separation between MTL and SRR.