Dental tissues have the unique property of recording their development history as histological growth markers. Animal studies have shown that many stress events (birth, weaning, infections) can generate a chemical signature. Enamel and dentin offer a retrospective view of significant events occurring in growth but are limited in time to the end of the permanent dentition growth and development. Recent improvements in cementum histological analysis offer new perspectives for analyzing stressors and life history events throughout life. This chapter tests the hypothesis that pregnancy may disrupt acellular cementum (AC) deposits visible in the mineralized matrix, using light microscopy, Raman spectrometry, and scanning electron microscopy equipped with an EDS probe. Two human samples with known age at pregnancies demonstrated that accentuated AC increments can be identified and precisely matched to these events. In both samples, these AC variations were the most outstanding optically and chemically. This is notable since such a method’s ultimate purpose is to identify fertility events in archaeological samples blindly.