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1 results

  • Effects of a biologically active fraction from Melia volkensii on Culex quinquefasciatus

  • L. W. Irungu, R. W. Mwangi
  • Journal:
    Insect Science and Its Application / Volume 16 / Issue 2 / June 1995
    Published online by Cambridge University Press:
    19 September 2011, pp. 159-162
    Print publication:
    June 1995

  • A chromatographically enriched fraction designated Fraction B from dry fruits of the plant Melia volkensii (family Meliaceae) was evaluated with the objective of determining its toxic and growth inhibiting effects on the larvae and adults of Culex quinquefasciatus mosquitoes. The fraction was purified from a crude methanolic extract by cold precipitation and elution of the precipitate dissolved in a hexane-ethyl acetate solvent system through a silica gel column. Larval treatments involved rearing the larval stages in water containing the fraction at concentrations of between 5 and 200 ppm. The LC50 for this fraction was found to be 34.72 μg/ml in 48 h. Second instar larvae were found to be more susceptible to fraction B when compared to fourth instar larvae. All fourth instar larvae that survived the treatment moulted into larval-pupal intermediates that were short-lived. The extract was also found to be an oviposition deterrent at a concentration of 20 ppm and above. It is concluded that M. volkensii extract has potential in the control of Culex quinquefasciatus.