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induction génétique

Aquatic Living Resources (1)

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Induced triploidy in the common carp (Cyprinus carpio L.): a comparison of two methods
Otomar Linhart, Martin Flajshans, Pavel Kvasnieka
Journal:

Aquatic Living Resources / Volume 4 / Issue 3 / July 1991

Published online by Cambridge University Press:

15 July 1991, pp. 139-145

Print publication:

July 1991
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Cold shocks and hydrostatic pressure shocks, respectively, were applied for the induction of bothtriploidy and tetraploidy in the common carp. Cold shocks of 0-2 °C, lasting 40 minutes and starting 2–5 minutes after the activation of gametes and hydrostatic pressure shocks with 1-3-5 minute exposures at 49.03–56.88 MPa (i. e. 7 116-8 225 psi – pounds per square inch), starting 5 minutes after the activation of gametes gave the best results. The tetraploidy induction by cold shocks or hydrostatic pressure shocks resulted either in negligible (1.54%), or in zero yields, respectively. The applicability of particular types of ploidy manipulation and the standardization of shock initiation were discussed.