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2 results

  • 1 - Introduction

  • Michele Goodwin
  • Book:
    Policing the Womb
    Published online:
    07 February 2020
    Print publication:
    12 March 2020, pp 1-11

  • Summary

    This is not a work of fiction, although I wish it were. Some of the cases described here could recall the imagery evoked by Mary Shelly, author of Frankenstein; or, The Modern Prometheus, who tells a horror story about a young rogue scientist who creates an unsightly monster through clandestine, aberrant experimentation. Although Frankenstein is the name of the monster’s creator, Dr. Victor Frankenstein, readers would be forgiven for debating who the real monster happens to be. In Policing the Womb, the story of Marlise Muñoz comes to mind. Brain-dead, decomposing in a Texas hospital, forced by state legislation to gestate a barely developing fetus while her body decays and the anomalies in the fetus mount. Eventually, it will be reported that the fetus is hydrocephalic, which means severe brain damage in this case and water or fluid developing on its brain. Medical reports will also show that the fetus is not developing its lower extremities. The state knows brain death is irreversible.

  • Efficiency of different incubation systems for the in vitro production of bovine embryos

  • Camila M. Cavalcanti, Iana S. Campelo, Mirelly M.A.S. Silva, João V.S. Albuquerque, Luciana M. Melo, Vicente J.F. Freitas
  • Journal:
    Zygote / Volume 26 / Issue 4 / August 2018
    Published online by Cambridge University Press:
    12 September 2018, pp. 314-318

  • This study aimed to compare the efficiency of different incubation systems for in vitro embryo production in bovine. Oocytes/embryos were cultured in three incubators: conventional – CONV, mini bench – MINI and portable – PORT. After in vitro maturation (IVM), oocytes were verified for maturation rate. The remaining structures were submitted to in vitro fertilization and culture to verify cleavage (day 2) and blastocyst (day 7) rates. Reactive oxygen species (ROS) were evaluated in post-IVM oocytes and embryos (days 2 and 7) using arbitrary fluorescence units (AFUs). No significant difference (P>0.05) was observed for maturation rate. The CONV system (74.0%) produced the highest cleavage rate (P<0.05) when compared with PORT (59.5%), but similar (P>0.05) to MINI (65.0%). The same pattern and differences were observed for blastocyst rate: CONV (33.3%), MINI (32.3%) and PORT (21.9%). ROS levels were not different (P>0.05) in post-IVM oocytes: CONV (35.6±4.5), MINI (29.4±4.0) and PORT (35.6±4.5). For day-2 embryos, ROS levels were higher (P<0.05) in MINI (44.2±3.1) in comparison with CONV (27.7±3.7) and PORT (33.3±3.2). No significant difference (P>0.05) was observed in blastocysts. In conclusion, although it produced high ROS levels at day 2 of culture, the MINI system was as efficient as the CONV system for blastocyst production. This option may be an interesting and economical for the in vitro embryo industry.