When herbicide concentration was constant, absorption of 14C-glyphosate increased with increasing droplet size (326 to 977 μm). Amount of 14C-glyphosate translocated away from the treated area, expressed as percent of absorbed, increased as droplet size decreased. Herbicide concentration of the droplet was more important than droplet number or droplet size in determining glyphosate absorption and translocation. Absorption and translocation increased with increasing herbicide concentration regardless of whether droplet size or number was altered in conjunction with herbicide concentration. This relationship explained why low spray volume (increased herbicide concentration) increased herbicide efficacy. The concentration gradient between droplet and leaf, rather than droplet coverage, was the primary mechanism responsible for the observed effect. Large droplets caused localized tissue injury, which may have caused decreased translocation.