In order to safeguard the welfare of laboratory-housed non-human primates, refinement techniques should be applied to every aspect of the life of animals used in the laboratory, from birth to death, with the aim of both minimising harm and maximising well-being. In this second part of a three-part review we summarise published information on housing and husbandry practices, and describe ways to minimise contingent inhumanity associated with the use of primates in laboratories and their breeding and supply (where inhumanity is defined as the infliction of distress). We also discuss methods by which the welfare of these animals can be maximised on a day-to-day basis. The principles of enrichment, aspects of the housing environment, social and physical enrichment and acquisition are discussed. Refinement of the influence of humans and experimental procedures are discussed in Parts I and III of this review, respectively.