The aim of this article is to make clear that, although men largely dominated the institutions of the Holy Roman Empire, beyond these constitutional institutions we can find many examples of women's agency. In particular, women of noble and princely families assumed political roles, both in relation to territories and to the empire as a whole. While it would not be correct to reinterpret the Holy Roman Empire as a “realm of ladies,” it seems clear that the empire, as a communicative context and dynastic network, was constituted with the participation of elite women, and that women were important for the ritual perpetuation of the constitution of the empire. In short, it was not only law and the constitution and the actions of men that held the empire together, but also the actions of women, who helped shape networks and politics just as they influenced the transfer of knowledge and culture.