Understanding seed biology and onset of germination requirements is a key point for designing effective weed management programs. Gibberellins (GAs) are known to play a role in onset of germination of several species. Onset of germination often requires an increase in de novo GA content or an increase in sensitivity to GAs. Reduced germination of seeds incubated in solutions containing compounds that inhibit GA synthesis provide evidence that GAs are required to trigger germination. Trinexapac-ethyl (TE), a GA synthesis inhibitor, is frequently used as a plant growth regulator in crop production. However, to the best of our knowledge, TE has not been used to study the requirement of GAs in onset of germination. Germination studies were conducted using seeds of artichoke thistle and common teasel under a range of TE concentrations (0 to 500 μM TE), a combined solution of 125 μM TE + 100 μM GA3 for artichoke, and 250 μM TE + 100 μM GA3 for common teasel. Germination tests were conducted at 20/10 C (12-h thermoperiod) in darkness for artichoke and at constant 15 C with 12 h of light for common teasel. Germination of artichoke in 125 μM TE was reduced to 47% when compared with the check (95%), but the combined TE + GA3 solution maintained germination at 84%. Germination of common teasel in 250 μM TE was reduced to 10% when compared with the check (91%), and the combined TE + GA3 solution increased germination to 63%. These results demonstrate the utility of TE to study the role of GAs in onset of germination. This novel use of TE is a valuable option to study germination requirements of weed species, and therefore contributes to the design of weed management programs.